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Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 9 — Sep. 8, 2008

Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology

Toyohiko Yamauchi, Hidenao Iwai, Mitsuharu Miwa, and Yutaka Yamashita  »View Author Affiliations


Optics Express, Vol. 16, Issue 16, pp. 12227-12238 (2008)
http://dx.doi.org/10.1364/OE.16.012227


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Abstract

We have developed a Linnik-type interference microscope provided with a low-coherent light source to obtain topographic images of an intact cellular membrane on a nanometer scale. Our technique is based on measurement of the interference between light reflected from the cell surface and a reference beam. The results show full field surface topography of cultured cells and reveal an intrinsic membrane motion of tens of nanometers.

© 2008 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(110.3175) Imaging systems : Interferometric imaging

ToC Category:
Microscopy

History
Original Manuscript: May 14, 2008
Revised Manuscript: July 1, 2008
Manuscript Accepted: July 28, 2008
Published: July 31, 2008

Virtual Issues
Vol. 3, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Toyohiko Yamauchi, Hidenao Iwai, Mitsuharu Miwa, and Yutaka Yamashita, "Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology," Opt. Express 16, 12227-12238 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-16-12227


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