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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 9 — Sep. 8, 2008

Quantification of three-dimensional dynamics of intercellular geometry under mechanical loading using a weighted directional adaptive-threshold method

Nikola Kojic, Austin Huang, Euiheon Chung, Daniel Tschumperlin, and Peter T. C. So  »View Author Affiliations

Optics Express, Vol. 16, Issue 16, pp. 12403-12414 (2008)

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Capturing and quantifying dynamic changes in three-dimensional cellular geometries on fast time scales is a challenge because of mechanical limitations of imaging systems as well as of the inherent tradeoffs between temporal resolution and image quality. We have combined a custom high-speed two-photon microscopy approach with a novel image segmentation method, the weighted directional adaptive-threshold (WDAT), to quantify the dimensions of intercellular spaces of cells under compressive stress on timescales previously inaccessible. The adaptation of a high-speed two-photon microscope addressed the need to capture events occurring on short timescales, while the WDAT method was developed to address artifacts of standard intensity-based analysis methods when applied to this system. Our novel approach is demonstrated by the enhanced temporal analysis of the three-dimensional cellular and extracellular deformations that accompany compressive loading of airway epithelial cells.

© 2008 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(100.3008) Image processing : Image recognition, algorithms and filters

ToC Category:
Image Processing

Original Manuscript: June 6, 2008
Revised Manuscript: July 27, 2008
Manuscript Accepted: July 28, 2008
Published: August 1, 2008

Virtual Issues
Vol. 3, Iss. 9 Virtual Journal for Biomedical Optics

Nikola Kojic, Austin Huang, Euiheon Chung, Daniel Tschumperlin, and Peter T. So, "Quantification of three-dimensional dynamics of intercellular geometry under mechanical loading using a weighted directional adaptive-threshold method," Opt. Express 16, 12403-12414 (2008)

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