OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 11 — Oct. 22, 2008

Generation of multiple Bessel beams for a biophotonics workstation

T. Čižmár, V. Kollárová, X. Tsampoula, F. Gunn-Moore, W. Sibbett, Z. Bouchal, and K. Dholakia  »View Author Affiliations

Optics Express, Vol. 16, Issue 18, pp. 14024-14035 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (898 KB) Open Access

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a simple method using an axicon and spatial light modulator to create multiple parallel Bessel beams and precisely control their individual positions in three dimensions. This technique is tested as an alternative to classical holographic beam shaping commonly used now in optical tweezers. Various applications of precise control of multiple Bessel beams are demonstrated within a single microscope giving rise to new methods for three-dimensional positional control of trapped particles or active sorting of micro-objects as well as “focus-free” photoporation of living cells. Overall this concept is termed a ‘biophotonics workstation’ where users may readily trap, sort and porate material using Bessel light modes in a microscope.

© 2008 Optical Society of America

OCIS Codes
(090.1970) Holography : Diffractive optics
(110.0110) Imaging systems : Imaging systems
(140.3300) Lasers and laser optics : Laser beam shaping
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: July 16, 2008
Revised Manuscript: August 18, 2008
Manuscript Accepted: August 21, 2008
Published: August 25, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Tomas Cižmár, V. Kollárová, X. Tsampoula, F. Gunn-Moore, W. Sibbett, Z. Bouchal, and K. Dholakia, "Generation of multiple Bessel beams for a biophotonics workstation," Opt. Express 16, 14024-14035 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. Durnin, J. J. Miceli, and J. Eberly, "Diffraction-free beams," Phys. Rev. Lett. 58, 1499-1501 (1987). [CrossRef] [PubMed]
  2. Z. Bouchal, J. Wagner, and M. Chlup, "Self-reconstruction of a distorted nondiffracting beam," Opt. Commun. 151, 207-211 (1998). [CrossRef]
  3. V. Garcés-Chávez, D. Roskey, M. D. Summers, H. Melville, D. McGloin, E. M. Wright, and K. Dholakia, "Optical levitation in a Bessel light beam," Appl. Phys. Lett. 8, 4001-4003 (2004). [CrossRef]
  4. L. Paterson, E. Papagiakoumou, G. Milne, V. Garcés-Chávez, T. Briscoe, W. Sibbett, L. Dholakia, and A. Riches, "Passive optical separation within a �??nondiffracting�?? light beam," J. Biomed. Opt. 12(054017) (2007). [CrossRef] [PubMed]
  5. T. �?ižmár, V. Garcés-Chávez, K. Dholakia, and P. Zemánek, "Optical conveyor belt for delivery of submicron objects," Appl. Phys. Lett.  86, 174,101-1-174,101-3 (2005).
  6. T �?ižmár, V. Kollárová, Z. Bouchal, and P. Zemánek, "Sub-micron particle organization by self-imaging of non-diffracting beams," New. J. Phys. 8, 43 (2006). [CrossRef]
  7. C. Sheppard and A. Choudhury, "Annular pupils, radial polarization, and superresolution," Appl. Opt. 43(22), 4322 - 4327 (2004). [CrossRef]
  8. X. Tsampoula, V. Garcés-Chávez, M. Comrie, D. Stevenson, B. Agate, C. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam,"Appl. Phys. Lett.  91(5), 053,902 (2007).
  9. S. Schmid, G. Thalhammer, K. Winkler, F. Lang, and J. Denschlag, "Long distance transport of ultracold atoms using a 1D optical lattice," New J. Phys. 8, 159 (2006). [CrossRef]
  10. B. P. S. Ahluwalia, X.-C. Yuan, S. H. Tao, J. Bu, H. Wang, X. Peng, and H. B. Niu, "Microfabricated-compositehologram-enabled multiple channel longitudinal optical guiding of microparticles in nondiffracting core of a Bessel beam array," Appl. Phys. Lett. 87, 084,104 (2005). [CrossRef]
  11. Z. Jiang, Q. Lu, and Z. Liu, "Propagation of apertured Bessel beams," Appl. Opt. 34(31), 7183 - 7185 (1995). [CrossRef]
  12. Z. Bouchal, "Controlled spatial shaping of nondiffracting patterns and arrays," Opt. Lett. 27(16), 1376 - 1378 (2002). [CrossRef]
  13. Z. Bouchal, "Vortex array carried by a pseudo-nondiffracting beam," J. Opt. Soc. Am. A 21(9), 1694 - 1702 (2004). [CrossRef]
  14. H. Little, C. Brown, V. Garcés-Chávez, W. Sibbett, and K. Dholakia, "Optical guiding of microscopic particles in femtosecond and continuous wave Bessel light beams," Opt. Express 12(11), 2560-2565 (2004). [CrossRef]
  15. J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, 1968).
  16. J. Lin, X. Yuan, S. Tao, and R. Burge, "Collinear superposition of multiple helical beams generated by a single azimuthally modulated phase-only element," Opt. Lett. 30(24), 3266 - 3268 (2005). [CrossRef]
  17. J. E. Curtis, B. A. Koss, and D. G. Grier, "Dynamic holographic optical tweezers," Opt. Commun. 207, 169-175 (2002). [CrossRef]
  18. V. Jarutis, R. Paškauskas, and A. Stabinis, "Focusing of Laguerre-Gaussian beams by axicon," Opt. Commun. 1841-4, 105-112 (2000). [CrossRef]
  19. O. Brzobohatỳ, T. �?ižmár, and P. Zemánek, "High quality quasi-Bessel beam generated by oblate-tip axicon," Opt. Express 16(17), 12688 - 12700 (2008).
  20. G. Milne, K. Dholakia, D. McGloin, K. Volke-Sepulveda, and P. Zemanek, "Transverse particle dynamics in a Bessel beam," Opt. Express 15(21), 13,972 - 13,987 (2007).
  21. C. T. A. Brown, D. J. Stevenson, X. Tsampoula, C. McDougall, A. A. Lagatsky, W. Sibbett, F. J. Gunn-Moore, and K. Dholakia, "Enhanced operation of femtosecond lasers and applications in cell transfection," Journal of Biophotonics (in press) (2008).
  22. D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14(16), 7125-7133 (2006). [CrossRef]
  23. L. E. Barrett, J. Y. Sul, H. Takano, E. J. Van Bockstaele, P. G. Haydon, and J. H. Eberwine, "Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor," Nature Methods 3, 455 - 460 (2006). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material

» Media 1: MOV (4109 KB)     
» Media 2: MOV (1290 KB)     
» Media 3: MOV (7037 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited