OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 11 — Oct. 22, 2008

Collagen and myosin characterization by orientation field second harmonic microscopy

Christophe Odin, Thomas Guilbert, Alia Alkilani, Olena P. Boryskina, Vincent Fleury, and Yann Le Grand  »View Author Affiliations


Optics Express, Vol. 16, Issue 20, pp. 16151-16165 (2008)
http://dx.doi.org/10.1364/OE.16.016151


View Full Text Article

Enhanced HTML    Acrobat PDF (2332 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Collagen and myosin fibrils are endogenous harmonophores that both give rise to Second Harmonic Generation (SHG). By combining four polarization SHG images provided by a scanning microscope, we show that the orientation of the principal axis of the nonlinear susceptibility tensor χ(2) can be determined for each pixel of the image. The ratio ρ=χ33/χ15 of the principal components of χ(2) of collagen and myosin was obtained with the same method, and found within the range 1.6–1.8 and 0.5–0.6 respectively. The orientation of the principal axis of χ(2) is shown to be correlated to the orientation of the fibrils themselves. This provides a straightforward method, which we call Orientation Field-Second Harmonic Microscopy (OF-SHM), to reconstruct orientation fields of fibrils at various scales and resolutions in different biological systems (from muscle sarcomere to the whole embryo).

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(160.1435) Materials : Biomaterials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 18, 2008
Revised Manuscript: August 26, 2008
Manuscript Accepted: August 29, 2008
Published: September 26, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Christophe Odin, Thomas Guilbert, Alia Alkilani, Olena P. Boryskina, Vincent Fleury, and Yann Le Grand, "Collagen and myosin characterization by orientation field second harmonic microscopy," Opt. Express 16, 16151-16165 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-20-16151


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. K. Kroy, "Elasticity, dynamics and relaxation in biopolymer networks," Curr. Opin. Colloin. Interface Sci. 11, 56-84 (2006). [CrossRef]
  2. G. A. Holzapfel "Computational Biomechanics of Soft Biological Tissue," in Encycl. Comput. Mech. E. Stein, R. de Borst and T. J. R. Hughes, eds., (John Wiley & Sons, Ltd, Chichester, 2004) 2, 605-635.
  3. K. Konig, "Multiphoton microscopy in life sciences," J. Microsc. 200, 83-104 (2000). [CrossRef] [PubMed]
  4. W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic : multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003). [CrossRef] [PubMed]
  5. P. J. Campagnola and L. M. Loew, "Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms," Nat. Biotechnol. 21, 1356-1360 (2003). [CrossRef] [PubMed]
  6. D. Debarre, A.-M. Pena, W. Supatto, T. Boulesteix, M. Strupler, M.-P. Sauviat, J.-L. Martin, M.-C. Schanne- Klein and E. Beaurepaire, "Second-and third-harmonic generation microscopies for the structural imaging of intact tissues, Med. Sci. 22, 845-850 (2006).
  7. V. Le Floc'h, S. Brasselet, J.-F. Roch, and J. Zyss, "Monitoring of Orientation in Molecular Ensembles by Polarization Sensitive Nonlinear Microscopy," J. Phys. Chem. B 107, 12403-12410 (2003). [CrossRef]
  8. S. Brasselet, V. Le Floch, F. Treussart, J.-F. Roch, J. Zyss, E. Botzung-Appert, and A. Ibanez, "In Situ Diagnostics of the Crystalline Nature of Single Organic Nanocrystals by Nonlinear Microscopy," Phys. Rev. Lett. 92, 207401- 207404 (2004). [CrossRef] [PubMed]
  9. S. Brasselet, J. Zyss, "Nonlinear polarimetry of molecular crystals down to the nanoscale," C. R. Physique R. Adv. Cryst. Opt. 8, 165-179 (2007).
  10. P. Stoller, K. M. Reiser, P. M. Celliers, and A. M. Rubenchik, "Polarization-Modulated Second Harmonic Generation in Collagen," Biophys. J. 82, 3330-3342 (2002).
  11. S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun. "Studies of |(2)/|(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy," Biophys. J. 86, 3914- 3922 (2004). [CrossRef] [PubMed]
  12. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman and W. W. Webb. "Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation," Proc. Natl. Acad. Sci. USA. 100,7075-7080 (2003). [CrossRef] [PubMed]
  13. A. Zoumi, A. Yeh, and B. J. Tromberg, "Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence," Proc. Nat. Acad. Sc. 20, 11014-11019 (2002). [CrossRef]
  14. T. Boulesteix, E. Beaurepaire, M. Sauviat, and M.-C. Schanne-Klein, "Second-harmonic microscopy of unstained living cardiac myocytes:measurements of sarcomere length with 20-nm accuracy," Opt. Lett. 29, 2031-2033 (2004). http://www.opticsinfobase.org/abstract.cfm?URI=ol-29-17-2031 [CrossRef] [PubMed]
  15. S. V. Plotnikov, A. C. Millard, P. J. Campagnola, and W. A. Mohler, "Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres," Biophys J. 90,693-703 (2006). [CrossRef]
  16. S. T. Jiang, "Contribution of Muscle Proteinases to Meat Tenderization," Proc. Natl. Sci. Council, ROC, Part B 22, 97-107 (1998).
  17. C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, "Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy," J. Microsc. 229, 32-38 (2008). [CrossRef] [PubMed]
  18. S. Roth and I. Freund, "Second harmonic generation in collagen," J. Chem. Phys. 70, 1637-1643(1979). [CrossRef]
  19. R. M. Williams, W. R. Zipfel, and W. W. Webb, "Interpreting Second-Harmonic Generation Images of Collagen I Fibrils," Biophys. J. 88, 1377-1386 (2005). [CrossRef]
  20. P. Fratzl, "Cellulose and collagen: from fibres to tissues," Curr. Op. Coll. Int. Sc. 8, 32-39 (2003). [CrossRef]
  21. D. A. Kleinman, "Nonlinear Dielectric Polarization in Optical Media," Phys. Rev. 126, 1977-1979 (1962). [CrossRef]
  22. F. Tiaho, G. Recher, and D. Rouede, "Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy," Opt. Express 15, 12286-12295 (2007), http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-19-12286. [CrossRef] [PubMed]
  23. X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, "Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma," Opt. Express 16, 1846-1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
  24. C. Odin, "NMR studies of Phase Transitions," Ann. Rep. NMR Spectr., G. Webb ed (Elsevier/North-Holland, Amsterdam, 2006) 59, 117-205. [CrossRef]
  25. L. Gao, L. Jin, P. Xue, J. Xu, Y. Wang, H. Ma, and D. Chen, "Reconstruction of complementary images in second harmonic generation microscopy," Opt. Express 14, 4727-4735 (2006), http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-11-4727. [CrossRef] [PubMed]
  26. H. Hamburger and H. L. Hamilton "A series of normal stages in the development of the chick embryo," J. Morphol. 88, 49-92 (1951). [CrossRef]
  27. K. V. Mardia and P. E. Jupp, Directional Statistics (John Wiley and Sons Ltd, Chichester, 2000).
  28. P. J. Elbischger, H. Bischof, P. Regitnig, and G. A. Holzapfel, "Automatic analysis of collagen fiber orientation in the outermost layer of human arteries," Pattern Anal Applic 7, 269-284 (2004).
  29. M. H. Stromer, D. E. Goll, R. B. Young, R. M. Robson, and F. C. Parrish, "Ultrastructural features of skeletal muscle differentiation and development," Jr. J. Anim Sci. 38, 1111-1141 (1974).
  30. A. Leray, L. Leroy, Y. Le Grand, C. Odin, A. Renault, V. Vie, D. Rouede, T. Mallegol, O. Mongin, M. H. V. Werts, and M. Blanchard-Desce, "Organization and orientation of amphiphilic push-pull chromophores deposited in Langmuir-Blodgett monolayers studied by second-harmonic generation and atomic force microscopy, Langmuir 20, 8165-8171 (2004). [CrossRef] [PubMed]
  31. O. P. Boryskina, Y. Le Grand, C. Odin, and V. Fleury, "The role of distribution and orientation of collagen fibers in tissue development: study by means of double imaging by two-photon excited fluorescence and second harmonic generation microscopy," Proc Europ. Microw. Assoc. 4, 255-259 (2008).
  32. M. L. Concha and R. J. Adams, "Oriented cell divisions and cellular morphogenesis in the zebrafish gastrula and neurula: a time-lapse analysis," Development 125, 983-994 (1998). [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited