OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 12 — Dec. 1, 2008

Spectroscopic ultrahigh-resolution full-field optical coherence microscopy

Arnaud Dubois, Julien Moreau, and Claude Boccara  »View Author Affiliations

Optics Express, Vol. 16, Issue 21, pp. 17082-17091 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (1563 KB) Open Access

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We have developed a full-field optical coherence microscopy system providing intensity-based tomographic images and spectroscopic information with ultrahigh spatial resolution. Local measurements of the backscattered light spectrum center of mass are achieved through short-time Fourier analysis of a stack of en face interferometric images acquired with a Linnik-type microscope. Using a halogen lamp as an illumination source enables us to achieve spectroscopic imaging over a wavelength range from 600 to 900 nm with a spatial resolution of ~1 µm. Absorption measurements of a colored gel are reported as a validation of the technique. Enhancement of tissue imaging contrast is demonstrated by imaging a Xenopus Laevis (African frog) tadpole ex vivo.

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.3170) Microscopy : Interference microscopy

ToC Category:

Original Manuscript: May 7, 2008
Revised Manuscript: June 12, 2008
Manuscript Accepted: June 19, 2008
Published: October 10, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Arnaud Dubois, Julien Moreau, and Claude Boccara, "Spectroscopic ultrahigh-resolution full-field optical coherence microscopy," Opt. Express 16, 17082-17091 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. L. Vabre, A. Dubois, and A. C. Boccara, "Thermal-light full-field optical coherence tomography," Opt. Lett. 27, 530-533 (2002). [CrossRef]
  2. A. Dubois, K. Grieve, G. Moneron, R. Lecaque, L. Vabre, and A. C. Boccara, "Ultrahigh-resolution full-field optical coherence tomography," Appl. Opt. 43, 2874 (2004). [CrossRef] [PubMed]
  3. A. Dubois, G. Moneron, and A. C. Boccara, "Thermal-light full-field optical coherence tomography in the 1.2 µm wavelength region," Opt. Commun. 266, 738-743 (2006). [CrossRef]
  4. K. Grieve, M. Paques, A. Dubois, J. Sahel, A.C. Boccara, J. F. Le Gargasson, "Ocular tissue imaging using ultrahigh-resolution full-field optical coherence tomography," Invest. Ophthalmol. Visual Sci. 45, 4126-4131 (2004). [CrossRef]
  5. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical Coherence Tomography," Science 254, 1178 (1991). [CrossRef] [PubMed]
  6. J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, and E. A. Swanson, "Optical biopsy and imaging using optical coherence tomography," Nature Med. 1, 970 (1995). [CrossRef] [PubMed]
  7. A. F. Fercher, "Optical coherence tomography," J. Biomed. Opt. 1, 157 (1996). [CrossRef]
  8. G. Moneron, A. C. Boccara, and A. Dubois, "Stroboscopic ultrahigh-resolution full-field optical coherence tomography," Opt. Lett. 30, 1351-1353 (2005). [CrossRef] [PubMed]
  9. K. Grieve, A. Dubois, M. Simonutti, M. Paques, J. Sahel, J. F. Le Gargasson, A. C. Boccara, "In vivo anterior segment imaging in the rat eye with high speed white light full-field optical coherence tomography," Opt. Express 13, 6286-6295 (2005). [CrossRef] [PubMed]
  10. M. D. Kulkarni and J. A. Izatt, CLEO �??96. Summaries of Papers Presented at the Conference on Lasers and Electro Optics 9 (96CH35899) 59-60 (1996).
  11. U. Morgner, W. Drexler, F. X. Kärtner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, "Spectroscopic optical coherence tomography, Opt. Lett. 25, 111-113 (2000). [CrossRef]
  12. R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C. K. Hitzenberger, M. Sticker, and A. F. Fercher, "Spectral measurement of absorption by spectroscopic frequency-domain optical coherence tomography," Opt. Lett. 25, 820-822 (2000). [CrossRef]
  13. K. G. Larkin, "Efficient nonlinear algorithm for envelope detection in white light interferometry," J. Opt. Soc. Am. A 13, 832-843 (1996). [CrossRef]
  14. D. Adler, T. Ko, P. Herz, and J. Fujimoto, "Optical coherence tomography contrast enhancement using spectroscopic analysis with spectral autocorrelation," Opt. Express 12, 5487-5501 (2004). [CrossRef] [PubMed]
  15. B. Hermann, K. Bizheva, A. Unterhuber, B. Považay, H. Sattmann, L. Schmetterer, A. Fercher, and W. Drexler, "Precision of extracting absorption profiles from weakly scattering media with spectroscopic time-domain optical coherence tomography," Opt. Express 12, 1677-1688 (2004). [CrossRef] [PubMed]
  16. C. Xu, P. Carney, and S. Boppart, "Wavelength-dependent scattering in spectroscopic optical coherence tomography," Opt. Express 13, 5450-5462 (2005). [CrossRef] [PubMed]
  17. C. Xu, D. Marks, M. Do, and S. Boppart, "Separation of absorption and scattering profiles in spectroscopic optical coherence tomography using a least-squares algorithm," Opt. Express 12, 4790-4803 (2004). [CrossRef] [PubMed]
  18. H. C. Van de Hulst, Light scattering by small particles, (Wiley, New York, 1957)
  19. A. Dubois, J. Selb, L. Vabre, and A. C. Boccara, "Phase measurements with wide-aperture interferometers," Appl. Opt. 39, 2326-2331 (2000) [CrossRef]
  20. A. Dubois, "Effects of phase change on reflection in phase-measuring interference microscopy," Appl. Opt. 43, 1503-1507 (2004) [CrossRef] [PubMed]
  21. C. Xu, C. Vinegoni, T. S. Ralston, W. Luo, W. Tan, and S. A. Boppart, "Spectroscopic spectral-domain optical coherence microscopy," Opt. Lett. 31, 1079-1081 (2006) [CrossRef] [PubMed]
  22. D. J. Faber, E. G. Mik, M. C. G. Alders, and T. G. van Leeuwen, "Light absorption of (oxy-)hemoglobin assessed by spectroscopic optical coherence tomography," Opt. Lett. 28, 1437-1439 (2003) [CrossRef]
  23. C. Xu, J. Ye, D. L. Marks, and S. A. Boppart, "Near-infrared dyes as contrast-enhancing agents for spectroscopic optical coherence tomography," Opt. Lett. 29,1647-1649 (2004) [CrossRef] [PubMed]
  24. G. P. Delacretaz, R. W. Steiner, L. O. Svaasand, H. J. Albrecht, and T. H. Meier, "Epidermal melanin absorption in human skin," in Laser-Tissue Interaction and Tissue Optics, Proc. SPIE 2624, 143-154 (1996). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited