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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 12 — Dec. 1, 2008

Spectroscopic ultrahigh-resolution full-field optical coherence microscopy

Arnaud Dubois, Julien Moreau, and Claude Boccara  »View Author Affiliations


Optics Express, Vol. 16, Issue 21, pp. 17082-17091 (2008)
http://dx.doi.org/10.1364/OE.16.017082


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Abstract

We have developed a full-field optical coherence microscopy system providing intensity-based tomographic images and spectroscopic information with ultrahigh spatial resolution. Local measurements of the backscattered light spectrum center of mass are achieved through short-time Fourier analysis of a stack of en face interferometric images acquired with a Linnik-type microscope. Using a halogen lamp as an illumination source enables us to achieve spectroscopic imaging over a wavelength range from 600 to 900 nm with a spatial resolution of ~1 µm. Absorption measurements of a colored gel are reported as a validation of the technique. Enhancement of tissue imaging contrast is demonstrated by imaging a Xenopus Laevis (African frog) tadpole ex vivo.

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 7, 2008
Revised Manuscript: June 12, 2008
Manuscript Accepted: June 19, 2008
Published: October 10, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Arnaud Dubois, Julien Moreau, and Claude Boccara, "Spectroscopic ultrahigh-resolution full-field optical coherence microscopy," Opt. Express 16, 17082-17091 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-21-17082


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