OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 12 — Dec. 1, 2008

Realtime Photoacoustic Microscopy of Murine Cardiovascular Dynamics

R.J. Zemp, L. Song, R. Bitton, K.K. Shung, and L.V. Wang  »View Author Affiliations

Optics Express, Vol. 16, Issue 22, pp. 18551-18556 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (586 KB) Open Access

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Non-invasive visualization of cardiovascular dynamics in small animals is challenging due to their rapid heart-rates. We present a realtime photoacoustic imaging system consisting of a 30-MHz ultrasound array transducer, receive electronics, a high-repetition-rate laser, and a multicore-computer, and demonstrate its ability to image optically-absorbing structures of the beating hearts of young athymic nude mice at rates of ~50 frames per second with 100 µm×25 µm spatial resolution. To our knowledge this is the first report of realtime photoacoustic imaging of physiological dynamics.

© 2008 Optical Society of America

OCIS Codes
(110.5100) Imaging systems : Phased-array imaging systems
(110.5120) Imaging systems : Photoacoustic imaging
(110.7170) Imaging systems : Ultrasound
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Imaging Systems

Original Manuscript: June 10, 2008
Revised Manuscript: August 8, 2008
Manuscript Accepted: August 10, 2008
Published: October 24, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

R. J. Zemp, L. Song, R. Bitton, K. K. Shung, and L. V. Wang, "Realtime Photoacoustic Microscopy of Murine Cardiovascular Dynamics," Opt. Express 16, 18551-18556 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. F. S. Foster, M. Y. Zhang, Y. Q. Zhou, G. Liu, J. Mehi, E. Cherin, K. A. Harasiewicz, B. G. Starkoski, L. Zan, D. A. Knapik, and S. L. Adamson, "A new ultrasound instrument for in vivo microimaging of mice," Ultrasound. Med. Biol. 28, 1165-1172 (2002). [CrossRef] [PubMed]
  2. H. F. Zhang, K. Maslov, G. Stoica, and L. H. V. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nat. Biotechnol. 24, 848-851 (2006). [CrossRef] [PubMed]
  3. M. H. Xu and L. H. V. Wang, "Photoacoustic imaging in biomedicine," Rev. Sci. Instrum. 77, - (2006).
  4. E. Zhang, and P. Beard, "Broadband ultrasound field mapping system using a wavelength tuned, optically scanned focused laser beam to address a Fabry Perot polymer film sensor," IEEE Trans. Ultrason. Ferroelectr. Freq. Contr. 53, 1330-1338 (2006). [CrossRef]
  5. M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol. 52, 1349-1361 (2007). [CrossRef] [PubMed]
  6. L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. H. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 1-3 (2007). [CrossRef]
  7. R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007). [CrossRef] [PubMed]
  8. R. Zemp, L. Song, R. Bitton, K. Shung, and L. Wang, "Realtime photoacoustic microscopy in vivo with a 30-MHz ultrasound array transducer," Opt. Express 16, 7915-7928 (2008). [CrossRef] [PubMed]
  9. J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Contr. 53, 224-236 (2006). [CrossRef]
  10. A. G. Richards, E. Simonson, and M. B. Visscher, "Electrocardiogram and Phonogram of Adult and Newborn Mice in Normal Conditions and Under the Effect of Cooling, Hypoxia and Potassium," Am. J. Physiol 174, 293-298 (1953). [PubMed]
  11. K. Kramer, S. A. B. E. Vanacker, H. P. Voss, J. A. Grimbergen, W. J. F. Vandervijgh, and A. Bast, "Use of Telemetry to Record Electrocardiogram and Heart-Rate in Freely Moving Mice," J. Pharmacological Toxicological Meth. 30, 209-215 (1993). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

Supplementary Material

» Media 1: MOV (1769 KB)     
» Media 2: MOV (3189 KB)     

« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited