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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 2 — Feb. 10, 2009

Nonlinear optical endoscope based on a compact two axes piezo scanner and a miniature objective lens

R. Le Harzic, M. Weinigel, I. Riemann, K. König, and B. Messerschmidt  »View Author Affiliations

Optics Express, Vol. 16, Issue 25, pp. 20588-20596 (2008)

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We report on a nonlinear optical endoscope that adopts a hollow core photonic crystal fiber for single-mode illumination delivery and a multimode one for signal collection. Femtosecond laser pulses up to 100 mW can be delivered at a centered wavelength of 800 nm. The two-photon fluorescence response of our system is shown to have axial and lateral resolutions of 5.8um and 0.6um respectively. Fluorescence detection was obtained at different wavelengths between 790 and 840 nm which could allow SHG detection for example. The maximal field-of-view of the acquired images is 420 µm×420 µm. Detection efficiency is greater by using an avalanche photodiode in comparison to a photo multiplier tube. Results presented here demonstrate the ability of the system to resolve cellular details and the potential of the device for future in vivo imaging diagnosis

© 2008 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(110.0180) Imaging systems : Microscopy
(110.2350) Imaging systems : Fiber optics imaging
(110.2760) Imaging systems : Gradient-index lenses
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 10, 2008
Revised Manuscript: November 5, 2008
Manuscript Accepted: November 12, 2008
Published: November 26, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

R. Le Harzic, M. Weinigel, I. Riemann, K. König, and B. Messerschmidt, "Nonlinear optical endoscope based on a compact two axes piezo scanner and a miniature objective lens," Opt. Express 16, 20588-20596 (2008)

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