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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 2 — Feb. 10, 2009

Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams

K.J. Moh, X.-C. Yuan, J. Bu, S. W. Zhu, and Bruce Z. Gao  »View Author Affiliations


Optics Express, Vol. 16, Issue 25, pp. 20734-20741 (2008)
http://dx.doi.org/10.1364/OE.16.020734


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Abstract

We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which forms a virtual probe on the metal substrate. The intensity distribution at the back focal plane of the objective lens enables quantitative measurements to be made of the cell-substrate contact. The acquired data is then visualized in the form of a local refractive index map.

© 2008 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(180.5810) Microscopy : Scanning microscopy
(240.6680) Optics at surfaces : Surface plasmons
(260.5430) Physical optics : Polarization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 3, 2008
Revised Manuscript: November 21, 2008
Manuscript Accepted: November 23, 2008
Published: December 1, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Citation
K. J. Moh, X.-C. Yuan, J. Bu, S. W. Zhu, and Bruce Z. Gao, "Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams," Opt. Express 16, 20734-20741 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-25-20734


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