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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 2 — Feb. 10, 2009

Interferometric method for birefringence determination with a polarizing microscope

Leonaş Dumitraşcu, Irina Dumitraşcu, Dana-Ortansa Dorohoi, and Mihai Toma  »View Author Affiliations

Optics Express, Vol. 16, Issue 25, pp. 20884-20890 (2008)

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We present a new mathematical technique which can be used to determine the main refractive indices and the birefringence of an anisotropic layer by using a polarizing microscope in conoscopic illumination. The values of the birefringence for the yellow radiation of a Na lamp are determined here for a Carpathian quartz sample, but the technique can also be applied to the study of other uniaxial substances such as liquid crystals, model membranes or biological tissues. The validity of the proposed method was tested by comparing the results with those obtained with a Rayleigh interferometer and by using the technology of channeled spectra.

© 2008 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(260.1440) Physical optics : Birefringence
(260.5430) Physical optics : Polarization

ToC Category:

Original Manuscript: July 28, 2008
Revised Manuscript: November 9, 2008
Manuscript Accepted: November 12, 2008
Published: December 3, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Leonas Dumitrascu, Irina Dumitrascu, Dana-Ortansa Dorohoi, and Mihai Toma, "Interferometric method for birefringence determination with a polarizing microscope," Opt. Express 16, 20884-20890 (2008)

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  1. L. A. Pajdzik and A. M. Glazer, "Three-dimensional birefringence imaging with a microscope tilting-stage. I. Uniaxial crystals," J. Appl. Cryst. 39, 326-337 (2006). [CrossRef]
  2. L. A. Pajdzik and A. M. Glazer, "Three-dimensional birefringence imaging with a microscope tilting stage. II. Biaxial crystals," J. Appl. Cryst. 39, 856-870 (2006). [CrossRef]
  3. M. A. Geday, W. Kaminsky, J. G. Lewis, and A. M. Glazer, "Images of absolute retardance L·Δn, using the rotating polariser method," J. Microsc. 198, 1-9 (2000). [CrossRef] [PubMed]
  4. W. Kaminsky, K. Claborn, and B. Kahr, "Polarimetric imaging of crystals," Chem. Soc. Rev. 33, 514-525 (2004). [CrossRef] [PubMed]
  5. Birefringence imaging system www.oxfordcryosystems.co.uk/downloads/Metripol brochure.pdf.
  6. A. Echalier, R. L. Glazer, V. Fülöp, and M. A. Geday, "Assessing crystallization droplets using birefringence," Acta Cryst. D 60, 696-702 (2004).
  7. M. Delibaş and D. O. Dorohoi, Practicum in Optics (in Romanian) (Ed. Univ. "Al. I. Cuza", Iaşi, 1999).
  8. G. Cone, Optics of anisotropic media (in Romanina) (Ed. Tehnica, Bucureşti, 1990).
  9. D. O. Dorohoi, Optics (in Romanian) (Ed. "Şt. Procopiu", Iaşi, 1995).
  10. V. Pop, Bases of Optics (in Romanian, (Ed. Univ. "Al. I. Cuza", Iaşi, 1988).
  11. M. Born and E. Wolf, Principles of Optics (Cambridge University Press, 1999).
  12. L. Dumitraşcu, I. Dumitraşcu, D. O. Dorohoi, D. Dimitriu, M. Aflori, and G. Apreutesei, Complementary Physics for PHD students (in Romanian), vol. I, (Ed. TehnoPress, Iaşi, 2007).
  13. http://www.creative.com.
  14. http:// www.mackichan.com.
  15. V. Pop, D. O. Dorohoi, and E. Cringeanu, "A New method for determining birefringence dispersion," J. Macromol. Sci. Phys. B33, 373-385 (1994).
  16. D. O. Dorohoi and M. Postolache, "The birefringence dispersion of poly-(phenyl) methacrylic ester of cetyl oxy benzoic acid, determined from channeled spectra," J. Macromol. Sci. Phys. 40, 239-249 (2001). [CrossRef]
  17. I. Dumitrascu, L. Dumitrascu, and D. O. Dorohoi, "The influence of the external field on the birefringence of the nematic liquid crystalline layer," J. Optoelectron. Adv. M. 8, 1028-1032 (2006).
  18. I. Diaconu, N. M. Puica, D. O. Dorohoi, and M. Aflori, "Birefringence dispersion of N-(4-methoxybenzilidene-4-butylaniline (MBBA) determined from channeled spectra," Spetrochim. Acta A 68, 536-541 (2007). [CrossRef]
  19. D. A. M. Androne, L. Dumitrascu, I. Horga, and D. O. Dorohoi, "Main refractive indices and birefringence of feldspars from Romanian pergmatites," Bull. Politechnical Inst. Iasi 55,137-141 (2006).
  20. D. M. A. Androne, D. O. Dorohoi, and D. Timpu, "Physical methods of identification of the feldspars from granite pergmatites," Romanian J. Phys. 53, 263-269 (2008).

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