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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 2 — Feb. 10, 2009

Polarization distortion effects in polarimetric two-photon microscopy

Peter Schön, Fabiana Munhoz, Alicja Gasecka, Sophie Brustlein, and Sophie Brasselet  »View Author Affiliations


Optics Express, Vol. 16, Issue 25, pp. 20891-20901 (2008)
http://dx.doi.org/10.1364/OE.16.020891


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Abstract

We present a global analysis of experimental factors affecting polarization responses in two-photon inverted microscopy. The role of reflection optics and high numerical aperture focusing is investigated in two-photon fluorescence, which can be extended to other nonlinear processes. We show that both effects strongly distort polarization responses and can lead to misleading extraction of molecular order information from polarimetric measurements. We describe a model accounting for these effects and develop a calibration technique for the determination of polarization parameters in the sample plane using two-photon fluorescence polarimetry in liquids.

© 2008 Optical Society of America

OCIS Codes
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 13, 2008
Revised Manuscript: September 23, 2008
Manuscript Accepted: September 24, 2008
Published: December 3, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Peter Schön, Fabiana Munhoz, Alicja Gasecka, Sophie Brustlein, and Sophie Brasselet, "Polarization distortion effects in polarimetric two-photon microscopy," Opt. Express 16, 20891-20901 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-25-20891


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