Engineering the collected field for single-molecule orientation determination

doi:10.1364/OE.16.003660

Engineering the collected field for single-molecule orientation determination

Zbigniew Sikorski and Lloyd M. Davis »View Author Affiliations

Optics Express, Vol. 16, Issue 6, pp. 3660-3673 (2008)
http://dx.doi.org/10.1364/OE.16.003660

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Abstract

We theoretically investigate the use of spatial light modulators (SLMs) for transformation of the collected fluorescence field in a high numerical aperture confocal microscope, for improved molecular orientation determination in single-molecule spectroscopy. The electric vector field in the back aperture of the microscope objective is calculated using the Weyl representation and taking into account components emitted at angles above the critical angle of the coverglass-immersion fluid interface. The coherently imaged fluorescence undergoes spatially-dependent phase and polarization transformation by the SLMs, before it passes to a polarization beamsplitter, and is subsequently focused onto two pinholes and single-photon detectors.

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(230.6120) Optical devices : Spatial light modulators
(260.1960) Physical optics : Diffraction theory
(320.7150) Ultrafast optics : Ultrafast spectroscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 21, 2007
Revised Manuscript: February 26, 2008
Manuscript Accepted: February 27, 2008
Published: March 5, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Zbigniew Sikorski and Lloyd M. Davis, "Engineering the collected field for single-molecule orientation determination," Opt. Express 16, 3660-3673 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-6-3660

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References

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