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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 4 — Apr. 23, 2008

Optics InfoBase > Virtual Journal for Biomedical Optics > Volume 3 > Issue 4 > Page 3818

Characterizing phase-separated microstructure of polymeric blended membrane using combined multiphoton and reflected confocal imaging

Hsin-Yuan Tan, Ming-Guo Lin, Wen-Chuo Hsiao, Sung-Jan Lin, Liang-Kun Wen, Wei-Liang Chen, Chen-Yuan Dong, and Tai-Horng Young  »View Author Affiliations


Optics Express, Vol. 16, Issue 6, pp. 3818-3827 (2008)
http://dx.doi.org/10.1364/OE.16.003818


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Abstract

In this study, we propose a novel, minimally-invasive multimodal optical imaging method which combines multiphoton and reflected confocal microscopy for characterizing three-dimensional phase-separated microstructure of polymeric nylon/chitosan blends. The multimodal image acquisition was performed on a Zeiss LSM 510 inverted microscope system using a ti-sapphire laser source. Differences in nonlinear optical signals between individual homopolymers were used to characterize the phase-separation phenomenon within the polymeric blends. We also used the reflected confocal signals for defining the interfacial boundaries of different refractive indices. Our work demonstrates that the proposed multimodal imaging modality can be used to provide the necessary microstructural information for characterizing the degrees of phase separation within polymeric blends.

© 2008 Optical Society of America

OCIS Codes
(160.5470) Materials : Polymers
(180.1790) Microscopy : Confocal microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: November 12, 2007
Revised Manuscript: February 20, 2008
Manuscript Accepted: February 26, 2008
Published: March 7, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Hsin-Yuan Tan, Ming-Guo Lin, Wen-Chuo Hsiao, Sung-Jan Lin, Liang-Kun Wen, Wei-Liang Chen, Chen-Yuan Dong, and Tai-Horng Young, "Characterizing phase-separated microstructure of polymeric blended membrane using combined multiphoton and reflected confocal imaging," Opt. Express 16, 3818-3827 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-6-3818


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References

  1. R. A. Kudva, H. Keskkula, and D. R. Paul, "Compatibilization of nylon 6/ABS blends using glycidyl methacrylate/methyl methacrylate copolymers," Polymer 39, 2447-2460 (1998). [CrossRef]
  2. J. Kressler, N. Higashida, T. Inoue, W. Heckmann, and F. Seitz, "Study of Polymer-Polymer Interfaces: A Comparison of Ellipsometric and TEM Data of PMMA/PS and PMMA/SAN Systems," Macromolecules 26, 2090-2094 (1993). [CrossRef]
  3. J. H. Wang, C. W. Wei, H. C. Liu, and T. H. Young, "Behavior of MG-63 cells on nylon/chitosan-blended membranes," J. Biomed. Mater. Res. A. 64, 606-615 (2003). [CrossRef] [PubMed]
  4. S. J. Lin, W. C. Hsiao, S. H. Jee, H. S. Yu, T. F. Tsai, J. Y. Lai, and T. H. Young, "Study on the effects of nylon-chitosan-blended membranes on the spheroid-forming activity of human melanocytes," Biomaterials 27, 5079-5088 (2006). [CrossRef] [PubMed]
  5. P. Achalla, J. McCormick, T. Hodge, C. Moreland, P. Esnault, A. Karim, and D. Raghavan, "Characterization of Elastomeric Blends by Atomic Force Microscopy," J. Polym. Sci. B 44, 492-503 (2006).
  6. B. Serrano, J. Baselga, J. Bravo, F. Mikes, L. Sese, I. Esteban, and I. F. Pierola, "Chemical Imaging of Phase-Separated Polymer Blends by Fluorescence Microscopy," J. Fluoresc. 10, 135 (2000). [CrossRef]
  7. A. Gupper, P. Wilhelm, M. Schmied, and E. Ingolic. "Morphology of a PA/PTFE blend studied by Raman imaging," Macromol. Symp. 184, 275-286 (2002). [CrossRef]
  8. A. Chris, X. Gu, D. B. Chase, and S. J. Stranick. "Near-Field Infrared Imaging and Spectroscopy of a Thin Film Polystyrene/Poly(ethyl acrylate) Blend," Appl. Spectrosc. 58, 257-263 (2004). [CrossRef]
  9. H. Jinnai, Y. Nishikawa, T. Koga, and T. Hashimoto, "Direct Observation of Three-dimensional Bicontinuous Structure Developed via Spinodal Decomposition," Macromolecules 28, 4782-4784 (1995). [CrossRef]
  10. A. E. Ribbe, "Laser scanning confocal microscopy in polymer science," Trends Polym. Sci. 5, 333-337 (1997).
  11. H. Jinnai, H. Kitagishi, K. Hamano, Y. Nishikawa, and Takahashi, "Effect of confinement on phase-separation processes in a polymer blend observed by laser scanning confocal microscopy," Phys. Rev. E. Stat. Nonlin. Soft. Matter. Phys. 67, 021801 (2003). [CrossRef] [PubMed]
  12. A. E. Ribbe, M. Hayashi, M. Weber, and T. Hashimoto, "Identification of the morphology of a thin film by fluorescence laser-scanning confocal microscopy," Polymer. 39, 7149-7151(1998). [CrossRef]
  13. K. B. Sung, C. Liang, M. Descour, T. Collier, M. Follen, and R. Richards-Kortum, "Fiber-optic confocal reflectance microscope with miniature objective for in vivo imaging of human tissues," IEEE Trans. Biomed. Eng. 49, 1168-1172 (2002). [CrossRef] [PubMed]
  14. J. D. Bhawalkar, J. Swiatkiewicz, S. J. Pan, J. K. Samarabandu, W. S. Liou, G. S. He, R. Berezney, P. C. Cheng, and P. N. Prasad, "Three-Dimensional Laser Scanning Two-Photon Fluorescence Confocal Microscopy of Polymer Materials using a new, efficient upconverting Fluorophore," Scanning 18, 562-566 (1996). [CrossRef] [PubMed]
  15. A. Zoumi, A. Yeh, and B. J. Tromberg, "Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence," P. Natl. Acad. Sci. U.S.A. 99, 11014-11019 (2002). [CrossRef]
  16. H. S. Lee, S. W. Teng, H. C. Chen, W. Lo, T. Y. Lin, L. L. Chiou, C. C. Jiang, and C. Y. Dong. "Imaging human bone marrow stem cell morphogenesis in PGA scaffold by multiphoton microscopy," Tissue. Eng. 12, 2835-2841 (2006). [CrossRef]
  17. I. Constantinidis, C. L. Stabler, R. Long, and A. Sambanis, "Noninvasive monitoring of a retrievable bioartificial pancreas in vivo," Ann. N. Y. Acad. Sci. 961, 298-301 (2002). [CrossRef] [PubMed]
  18. Y. W. Wang, Q. Wu, J. C. Chen, and G. Q. Chen, "Evaluation of three-dimensional scaffolds made of blends of hydroxyapatite and poly(3-hydroxybutyrate-co- 3-hydroxyhexanoate) for bone reconstruction," Biomaterials 26, 899-904 (2005). [CrossRef]
  19. D. M. Burland, R. D. Miller, and C. A. Walsh. "Second-order nonlinearity in poled-polymer systems," Chem. Rev. 94, 31-75 (1994). [CrossRef]
  20. J. Xu, J. Bao, B. H. Guo, H. Ma, T. L. Yun, L. Gao, G. Q. Chen, T. Iwata. "Imaging of nonlinear optical response in biopolyesters via second harmonic generation microscopy and its dependence on the crystalline structures," Polymer. 48, 348-355 (2007). [CrossRef]
  21. S. Plotnikov, A. C. Millard, P. J. Campagnola, and W. A. Mohler, "Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres," Biophys J. 90, 693-703 (2006). [CrossRef]
  22. N. Y. Ha, S. H. Han, D. W. Jeon, C. S. Jung, B. Park, H. Takezoe, and J. W. Wu, "Analysis of surface second-harmonic generation by orientational distribution function in a chiral polymer film," Phys. Rev. E. Stat. Nonlin. Soft. Matter. Phys. 72, 036601 (2005). [CrossRef] [PubMed]
  23. W. Tan, A. Sendermir-Urkmez, L. J. Fahrmer, R. Jamison, and D. Leckband, "Structual and functional optical imaging of three-dimensional engineered tissue development," Tissue. Eng. 10, 1747-1757 (2004). [CrossRef]
  24. F. L. Mi, "Synthesis and characterization of a novel chitosan-gelatin bioconjugate with fluorescence emission," Biomacromolecules 6, 975-987 (2005). [CrossRef] [PubMed]
  25. A. Dufresne, J. Y. Cavaille, D. Dupeyre, M. Garcia-Ramirez, and J. Romero, "Morphology, phase continuity and mechanical behavior of polyamide 6/chitosan blends," Polymer. 40, 1657-1666 (1999). [CrossRef]
  26. A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Nelson, and B. J. Tromberg. "Imaging wound healing using optical coherence tomography and multiphoton microscopy in an in vitro skin-equivalent tissue model," J. Biomed. Opt. 9, 248-253 (2004). [CrossRef] [PubMed]
  27. A. Diaspro, F. Pellistri, F. Federici, A. Gerbi, P. Ramoino, and M. Robello, "Evaluation of optical properties and micropatterning capabilities of a TPE microscope based on a compact confocal scanning head," Proc. SPIE 4262, 383-388(2001). [CrossRef]
  28. S. Tang, T. B. Krasieva, Z. Chen, B. J. Tromberg, "Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source," J. Biomed. Opt. 11, 020502 (2006). [CrossRef] [PubMed]

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