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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 4 — Apr. 23, 2008

Optics InfoBase > Virtual Journal for Biomedical Optics > Volume 3 > Issue 4 > Page 5000

Polarization anisotropy in fiber-optic second harmonic generation microscopy

Ling Fu and Min Gu  »View Author Affiliations


Optics Express, Vol. 16, Issue 7, pp. 5000-5006 (2008)
http://dx.doi.org/10.1364/OE.16.005000


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Abstract

We report the investigation and implementation of a compact second harmonic generation microscope that uses a single-mode fiber coupler and a double-clad photonic crystal fiber. Second harmonic polarization anisotropy through the fiber-optic microscope systems is quantitatively measured with KTP microcrystals, fish scale and rat tail tendon. It is demonstrated that the polarized second harmonic signals can be excited and collected through the single-mode fiber coupler to analyze the molecular orientations of structural proteins. It has been discovered that a double-clad photonic crystal fiber can preserve the linear polarization in the core, although a depolarization effect is observed in the inner cladding region. The feasibility of polarization anisotropy measurements in fiber-optic second harmonic generation microscopy will benefit the in vivo study of collagen-related diseases with a compact imaging probe.

© 2008 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.2350) Imaging systems : Fiber optics imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 11, 2008
Revised Manuscript: March 25, 2008
Manuscript Accepted: March 25, 2008
Published: March 27, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Ling Fu and Min Gu, "Polarization anisotropy in fiber-optic second harmonic generation microscopy," Opt. Express 16, 5000-5006 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-7-5000


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