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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 8 — Jul. 30, 2009

Autofluorescence imaging of NADH and flavoproteins in the rat brain: insights from Monte Carlo simulations.

Barbara L’ Heureux, Hirac Gurden, and Frédéric Pain  »View Author Affiliations

Optics Express, Vol. 17, Issue 12, pp. 9477-9490 (2009)

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There has been recently a renewed interest in using Autofluorescence imaging (AF) of NADH and flavoproteins (Fp) to map brain activity in cortical areas. The recording of these cellular signals provides complementary information to intrinsic optical imaging based on hemodynamic changes. However, which of NADH or Fp is the best candidate for AF functional imaging is not established, and the temporal profile of AF signals is not fully understood. To bring new theoretical insights into these questions, Monte Carlo simulations of AF signals were carried out in realistic models of the rat somatosensory cortex and olfactory bulb. We show that AF signals depend on the structural and physiological features of the brain area considered and are sensitive to changes in blood flow and volume induced by sensory activation. In addition, we demonstrate the feasibility of both NADH-AF and Fp-AF in the olfactory bulb.

© 2009 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 17, 2008
Revised Manuscript: January 30, 2009
Manuscript Accepted: March 9, 2009
Published: May 22, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Barbara L'Heureux, Hirac Gurden, and Frédéric Pain, "Autofluorescence imaging of NADH and flavoproteins in the rat brain: insights from Monte Carlo simulations," Opt. Express 17, 9477-9490 (2009)

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