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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 8 — Jul. 30, 2009

Multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscopy and its applications for real-time imaging

Takeo Minamikawa, Mamoru Hashimoto, Katsumasa Fujita, Satoshi Kawata, and Tsutomu Araki  »View Author Affiliations


Optics Express, Vol. 17, Issue 12, pp. 9526-9536 (2009)
http://dx.doi.org/10.1364/OE.17.009526


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Abstract

We developed a multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscope using a microlens array scanner for real-time molecular imaging. Parallel exposure of a specimen with light from two highly controlled picosecond mode-locked lasers (jitter of 30 fs through an electronic low-pass filter with 150 Hz bandwidth, point-by-point wavelength scan within 300 ms) and parallel detection with an image sensor enabled real-time imaging. We demonstrated real-time CARS imaging of polystyrene beads (frame rate of 30 fps), a giant multi-lamellar vesicle of dipalmitoylphosphatidylcholine (frame rate of 10 fps), and living HeLa cells (frame rate of 10 fps).

© 2009 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Microscopy

History
Original Manuscript: January 9, 2009
Revised Manuscript: February 13, 2009
Manuscript Accepted: March 2, 2009
Published: May 22, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Takeo Minamikawa, Mamoru Hashimoto, Katsumasa Fujita, Satoshi Kawata, and Tsutomu Araki, "Multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscopy and its applications for real-time imaging," Opt. Express 17, 9526-9536 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-12-9526


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