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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 8 — Jul. 30, 2009

High-resolution computed tomography with a compact soft x-ray microscope

Michael Bertilson, Olov von Hofsten, Ulrich Vogt, Anders Holmberg, and Hans M. Hertz  »View Author Affiliations


Optics Express, Vol. 17, Issue 13, pp. 11057-11065 (2009)
http://dx.doi.org/10.1364/OE.17.011057


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Abstract

Computed tomography based on high-resolution soft x-ray microscopy utilizes the natural contrast for biological specimens provided by the water window (λ=2.4-4.4 nm) and the high resolving power of zone plate objectives. It is capable of revealing the 3D structure of biological specimens at sub-visible-microscopic resolution. To date, the technique has only been available at synchrotron-based microscopes, which limits the researchers access. In the present paper we demonstrate high-resolution soft x-ray tomography with a laboratory zone-plate-based soft x-ray microscope. The specimen, a diatom mounted on a glass capillary, was reconstructed from a tilt series of 53 images covering 180° using a filtered back projection algorithm. The resolution of the tomogram was estimated to a half period of 140 nm using a differential-phase-residual method. Cryo-fixation, increased source brightness and extended-depth-of-focus objectives are important for pushing the resolution of compact systems for biological samples.

© 2009 Optical Society of America

OCIS Codes
(180.7460) Microscopy : X-ray microscopy
(340.0340) X-ray optics : X-ray optics
(260.6048) Physical optics : Soft x-rays
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Microscopy

History
Original Manuscript: February 17, 2009
Revised Manuscript: April 21, 2009
Manuscript Accepted: June 10, 2009
Published: June 18, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Michael Bertilson, Olov von Hofsten, Ulrich Vogt, Anders Holmberg, and Hans M. Hertz, "High-resolution computed tomography with a compact soft x-ray microscope," Opt. Express 17, 11057-11065 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-13-11057


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