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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse

Keisuke Isobe, Akira Suda, Masahiro Tanaka, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa  »View Author Affiliations


Optics Express, Vol. 17, Issue 14, pp. 11259-11266 (2009)
http://dx.doi.org/10.1364/OE.17.011259


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Abstract

We demonstrate two complementary types of microscopy using an identical setup for single-pulse coherent anti-Stokes Raman scattering (CARS) imaging, which employs an ultrabroadband laser pulse with a spectral bandwidth of 4800 cm-1 and enables the suppression of nonresonant CARS signals. One is a novel type of microscopy that uses spectral phase modulation for the selective excitation of a single Raman mode. The selective excitation is achieved by the modulated pulse focusing its difference-frequency spectrum into a narrow spectral region. Another type is Fourier-transform CARS (FT-CARS) microspectroscopy based on the measurement of the CARS spectrum obtained from the Fourier-transform of the interferometric autocorrelation (IAC) signal. Vibrational spectral imaging of chemical and biological samples is demonstrated using the two types of microscopy.

© 2006 OSA

OCIS Codes
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6300) Spectroscopy : Spectroscopy, Fourier transforms
(320.5540) Ultrafast optics : Pulse shaping
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: April 28, 2009
Revised Manuscript: May 28, 2009
Manuscript Accepted: June 1, 2009
Published: June 22, 2006

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Keisuke Isobe, Akira Suda, Masahiro Tanaka, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa, "Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse," Opt. Express 17, 11259-11266 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-14-11259


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