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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Speckle-field digital holographic microscopy

YongKeun Park, Wonshik Choi, Zahid Yaqoob, Ramachandra Dasari, Kamran Badizadegan, and Michael S. Feld  »View Author Affiliations


Optics Express, Vol. 17, Issue 15, pp. 12285-12292 (2009)
http://dx.doi.org/10.1364/OE.17.012285


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Abstract

The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

© 2009 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 4, 2009
Revised Manuscript: June 25, 2009
Manuscript Accepted: June 28, 2009
Published: July 6, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
YongKeun Park, Wonshik Choi, Zahid Yaqoob, Ramachandra Dasari, Kamran Badizadegan, and Michael S. Feld, "Speckle-field digital holographic microscopy," Opt. Express 17, 12285-12292 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-15-12285


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References

  1. E. Cuche, F. Bevilacqua, and C. Depeursinge, “Digital holography for quantitative phase-contrast imaging,” Opt. Lett. 24(5), 291–293 (1999). [CrossRef]
  2. G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett. 29(21), 2503–2505 (2004). [CrossRef] [PubMed]
  3. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett. 31(6), 775–777 (2006). [CrossRef] [PubMed]
  4. Y. K. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Diffraction phase and fluorescence microscopy,” Opt. Express 14(18), 8263–8268 (2006). [CrossRef] [PubMed]
  5. C. Mann, L. Yu, C. M. Lo, and M. Kim, “High-resolution quantitative phase-contrast microscopy by digital holography,” Opt. Express 13(22), 8693–8698 (2005). [CrossRef] [PubMed]
  6. Y.-K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A. 105(37), 13730–13735 (2008). [CrossRef] [PubMed]
  7. G. Popescu, Y.-K. Park, N. Lue, C. Best-Popescu, L. Deflores, R. R. Dasari, M. S. Feld, and K. Badizadegan, “Optical imaging of cell mass and growth dynamics,” Am. J. Physiol. Cell Physiol. 295(2), C538–C544 (2008). [CrossRef] [PubMed]
  8. G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt. 10(6), 060503 (2005). [CrossRef]
  9. Y. K. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Fresnel particle tracing in three dimensions using diffraction phase microscopy,” Opt. Lett. 32(7), 811–813 (2007). [CrossRef] [PubMed]
  10. G. Popescu, Y.-K. Park, W. Choi, R. R. Dasari, M. S. Feld, and K. Badizadegan, “Imaging red blood cell dynamics by quantitative phase microscopy,” Blood Cells Mol. Dis. 41(1), 10–16 (2008). [CrossRef] [PubMed]
  11. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. Magistretti, “Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy,” Opt. Express 13(23), 9361–9373 (2005). [CrossRef] [PubMed]
  12. F. Dubois, M. L. Requena, C. Minetti, O. Monnom, and E. Istasse, “Partial spatial coherence effects in digital holographic microscopy with a laser source,” Appl. Opt. 43(5), 1131–1139 (2004). [CrossRef] [PubMed]
  13. M. G. Somekh, C. W. See, and J. Goh, “Wide field amplitude and phase confocal microscope with speckle illumination,” Opt. Commun. 174(1-4), 75–80 (2000). [CrossRef]
  14. M. C. Pitter, C. W. See, and M. G. Somekh, “Full-field heterodyne interference microscope with spatially incoherent illumination,” Opt. Lett. 29(11), 1200–1202 (2004). [CrossRef] [PubMed]
  15. C. Ventalon and J. Mertz, “Quasi-confocal fluorescence sectioning with dynamic speckle illumination,” Opt. Lett. 30(24), 3350–3352 (2005). [CrossRef]
  16. J. Walker, “Non-scanning confocal fluorescence microscopy using speckle illumination,” Opt. Commun. 189(4-6), 221–226 (2001). [CrossRef]
  17. C. Fang-Yen, S. Oh, Y.-K. Park, W. Choi, S. Song, H. S. Seung, R. R. Dasari, and M. S. Feld, “Imaging voltage-dependent cell motions with heterodyne Mach-Zehnder phase microscopy,” Opt. Lett. 32(11), 1572–1574 (2007). [CrossRef] [PubMed]
  18. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007). [CrossRef] [PubMed]
  19. S. Inoue, and K. R. and Spring, Video Microscopy (Plenum Press, New York and London, 1997).
  20. G. Nomarski, “Microinterféromètre différentiel à ondes polarisées,” J. Phys. Radium 16, 9S–11S (1955).
  21. J. Goodman, Introduction to Fourier optics (Roberts & Co, 2005).

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