OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells

Pierre Bon, Guillaume Maucort, Benoit Wattellier, and Serge Monneret  »View Author Affiliations


Optics Express, Vol. 17, Issue 15, pp. 13080-13094 (2009)
http://dx.doi.org/10.1364/OE.17.013080


View Full Text Article

Enhanced HTML    Acrobat PDF (1097 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. In particular we consider the effect of a non spatially coherent source on the phase-image signal-to-noise ratio. Precise measurements of the phase-shift introduced by microscopic beads or giant unilamellar vesicles validate the principle and show the accuracy of the methods. Diffraction limited images of living COS-7 cells are then presented, with a particular focus on the membrane and organelle dynamics.

© 2009 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
(290.3030) Scattering : Index measurements

ToC Category:
Microscopy

History
Original Manuscript: April 24, 2009
Revised Manuscript: July 3, 2009
Manuscript Accepted: July 6, 2009
Published: July 16, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Pierre Bon, Guillaume Maucort, Benoit Wattellier, and Serge Monneret, "Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells," Opt. Express 17, 13080-13094 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-15-13080


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. F. Zernike, "Das Phasenkontrastverfahren bei der mikroskopischen Beobachtung," Z. Tech. Phys. 16, 454-457 (1935).
  2. G. Nomarski, "Nouveau Dispositif Pour l’Observation En Contraste De Phase Differentiel," J. Phys. Radium 16, S88-S88 (1955).
  3. P. Hariharan, "Achromatic Phase-Shifting for White-Light Interferometry," Appl. Opt. 35(34), 6823-6824 (1996). [CrossRef]
  4. C. J. Cogswell, N. I. Smith, K. G. Larkin and P. Hariharan, "Quantitative DIC microscopy using a geometric phase shifter," Society of Photo-Optical Instrumentation Engineers (SPIE) Conference Series, C. J. Cogswell, J. A. Conchello and T. Wilson, eds. (1997), pp. 72-81.
  5. Y. Xu, Y. x. Xu, M. Hui and X. Cai, "Quantitative surface topography determination by differential interference contrast microscopy," Opt. Precision Eng. 9, 226-229 (2001).
  6. M. R. Arnison, K. G. Larkin, C. J. R. Sheppard, N. I. Smith, and C. J. Cogswell, "Linear phase imaging using differential interference contrast microscopy," J. Microscopy 214(1), 7-12 (2004). [CrossRef]
  7. S. Bernet, A. Jesacher, S. Fürhapter, C. Maurer and M. Ritsch-Marte, "Quantitative imaging of complex samples by spiral phase contrast microscopy," Opt. Express 14(9), 3792-3805 (2006). [CrossRef]
  8. E. Cuche, F. Bevilacqua and C. Depeursinge, "Digital holography for quantitative phase-contrast imaging," Opt. Lett. 24(5), 291-293 (1999). [CrossRef]
  9. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb and C. Depeursinge, "Digital holographic microscopy: a noninvasive contrastimaging technique allowing quantitative visualization ofliving cells with subwavelength axial accuracy," Opt. Lett. 30(5), 468-470 (2005). [CrossRef]
  10. F. Charrière, A. Marian, F. Montfort, J. Kuehn, T. Colomb, E. Cuche, P. Marquet and C. Depeursinge, "Cell refractive index tomography by digital holographic microscopy," Opt. Lett. 31(2), 178-180 (2006). [CrossRef]
  11. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari and M. S. Feld, "Tomographic phase microscopy," Nature Methods 4, 717-719 (2007). [CrossRef] [PubMed]
  12. M. Born and E. Wolf, Principles of Optics, (Cambridge University Press, 1999).
  13. J. Zou, A. Sautivet, J. Fils, L. Martin, K. Abdeli, C. Sauteret and B. Wattellier, "Optimization of the dynamic wavefront control of a pulsed kilojoule/nanosecond-petawatt laser facility," Appl. Opt. 47(5), 704-710 (2008). [CrossRef]
  14. R. V. Shack and B. C. Platt, "Production and use of a lenticular Hartmann screen," J. Opt. Soc. Am. 61, 656 (1971).
  15. A. Barty, K. A. Nugent, D. Paganin and A. Roberts, "Quantitative optical phase microscopy," Opt. Lett. 23(11), 817-819 (1998). [CrossRef]
  16. C. J. Bellair, C. L. Curl, B. E. Allman, P. J. Harris, A. Roberts and L. M. D. Delbridge, "Quantitative phase amplitude microscopy IV: imaging thick specimens," J. Microscopy 214(1), 62-68 (2004). [CrossRef]
  17. C. Curl, C. J. Bellair, P. J. Harris, B. E. Allman, A. Roberts, K. A. Nugent and L. M. D. Delbridge, "Single cell volume measurement by Quantitative Phase Microscopy (QPM): A case study of Erythrocyte Morphology," Cellular Physiology Biochem. 17, 193-200 (2006). [CrossRef] [PubMed]
  18. J. Primot and L. Sogno, "Achromatic three-wave (or more) lateral shearing interferometer," J. Opt. Soc. Am. A 12(12), 2679 (1995). [CrossRef]
  19. J. Primot, "Theoretical description of Shack-Hartmann wave-front sensor," Opt. Commun. 222, 81-92 (2003). [CrossRef]
  20. J. Primot and N. Guérineau, "Extended Hartmann Test Based on the Pseudoguiding Property of a Hartmann Mask Completed by a Phase Chessboard," Appl. Opt. 39(31), 5715-5720 (2000). [CrossRef]
  21. S. Velghe, J. Primot, N. Guérineau, R. Haidar, S. Demoustier, M. Cohen and B. Wattellier, "Advanced wave-front sensing by quadri-wave lateral shearing interferometry," Proc. SPIE 6292, 62920E (2006).
  22. M. J. Weber, Handbook of Optical materials, (CRC Press, 2003).
  23. M. Angelova, S. Soléau, P. Méléard and P. Bothorel, "Preparation of giant vesicles by external AC electric fields. Kinetics and applications," in Trends in Colloid and Interface Science V, Steinkopff Darmstadt, eds., (1992), pp. 127-131.
  24. J. F. Nagle and S. Tristram-Nagle, "Structure of lipid bilayers," Biochim. Biophys. Acta 1469(3), 159-195 (2000).
  25. C. S. Chong and C. Colbow, "Light scattering and turbidity measurements on lipid vesicles," Biochim. Biophys. Acta 436,260-282 (1976). [CrossRef] [PubMed]
  26. F. C. Jensen, J. Anthony, R. V. G. Girardi and H. Koprowski (1964), "Infection of Human and Simian Tissue Cultures with Rous Sarcoma Virus," PNAS 52(1), 53-59 (1964). [CrossRef]
  27. A. Sergé, N. Bertaux, H. Rigneault and D. Marguet, "Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes," Nat. Methods 5, 687-695 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Multimedia

Multimedia FilesRecommended Software
» Media 1: AVI (14264 KB)      QuickTime
» Media 2: AVI (3890 KB)      QuickTime

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited