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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells

Pierre Bon, Guillaume Maucort, Benoit Wattellier, and Serge Monneret  »View Author Affiliations

Optics Express, Vol. 17, Issue 15, pp. 13080-13094 (2009)

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Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. In particular we consider the effect of a non spatially coherent source on the phase-image signal-to-noise ratio. Precise measurements of the phase-shift introduced by microscopic beads or giant unilamellar vesicles validate the principle and show the accuracy of the methods. Diffraction limited images of living COS-7 cells are then presented, with a particular focus on the membrane and organelle dynamics.

© 2009 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
(290.3030) Scattering : Index measurements

ToC Category:

Original Manuscript: April 24, 2009
Revised Manuscript: July 3, 2009
Manuscript Accepted: July 6, 2009
Published: July 16, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Pierre Bon, Guillaume Maucort, Benoit Wattellier, and Serge Monneret, "Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells," Opt. Express 17, 13080-13094 (2009)

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