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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Analyzing absorption and scattering spectra of micro-scale structures with spectroscopic optical coherence tomography

Ji Yi, Jianmin Gong, and Xu Li  »View Author Affiliations


Optics Express, Vol. 17, Issue 15, pp. 13157-13167 (2009)
http://dx.doi.org/10.1364/OE.17.013157


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Abstract

We demonstrate the feasibility of characterizing the absorption and scattering spectra of micron-scale structures in a turbid medium using a spectroscopic optical coherence tomography (SOCT) system with a bandwidth of 430–650nm. SOCT measurements are taken from phantoms composed of fluorescent microspheres. The absorption and scattering spectra are recovered with proper selections of spatial window width in the post processing step. Furthermore, we present an analysis using numerical OCT simulation based on full-wave solutions of the Maxwell’s Equation to elucidate the origination of the multiple peaks in the OCT image for a single microsphere. Finally, we demonstrate the possibility of identifying contrast agents concentrated in micron-sized scale in an SOCT image. Two different types of microspheres in gel phantom are discriminated in 3D volume based on their distinguished absorbent feature.

© 2009 Optical Society of America

OCIS Codes
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(290.1350) Scattering : Backscattering
(300.1030) Spectroscopy : Absorption

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 13, 2009
Revised Manuscript: June 22, 2009
Manuscript Accepted: July 10, 2009
Published: July 17, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Ji Yi, Jianmin Gong, and Xu Li, "Analyzing absorption and scattering spectra of micro-scale structures with spectroscopic optical coherence tomography," Opt. Express 17, 13157-13167 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-15-13157


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References

  1. U. Morgner, W. Drexler, F. X. Kärtner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, "Spectroscopic optical coherence tomography," Opt. Lett. 25,111-113 (2000). [CrossRef]
  2. D. J. Faber, E.G. Mik, M. C. G. Aalders, and T. G. van Leeuwen, "Light absorption of (oxy-)hemoglobin assessed by spectroscopic optical coherence tomography," Opt. Lett. 28, 1436-1438 (2003). [CrossRef] [PubMed]
  3. S. A. Boppart, M. E. Brezinski, C Pitris, and J. G. Fujimoto, "Optical Coherence Tomography for Neurosurgical Imaging of Human Intracortical Melanoma," Neurosurgery 43, 834-841 (1998). [CrossRef] [PubMed]
  4. Z. Yaqoob, E. Mc Dowell, J. Wu, X. Heng, J. Fingler, and C. Yang, "Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent," J. Biomed. Opt. 11, 054017 (2006). [CrossRef] [PubMed]
  5. K. D. Rao, M. A. Choma, S. Yazdanfar, A. M. Rollins, and J. A. Izatt, "Molecular contrast in optical coherence tomography by use of a pump-probe technique," Opt. Lett. 28, 340-342 (2003). [CrossRef] [PubMed]
  6. C. Yang, L. E. L. McGuckin., J. D. Simon, M. A. Choma, B. E. Applegate, and J. A. Izatt, "Spectral triangulation molecular contrast optical coherence tomography with indocyanine green as the contrast agent," Opt. Lett. 29, 2016-2018 (2004). [CrossRef] [PubMed]
  7. C. Xu, J. Ye, D. L. Marks, and S. A. Boppart, "Near-infrared dyes as contrast-enhancing agents for spectroscopic optical coherence tomography," Opt. Lett. 29, 1647-1649 (2004). [CrossRef] [PubMed]
  8. D. C. Adler, T. H. Ko, P. R. Herz, and J. G. Fujimoto, "Optical coherence tomography contrast enhancement using spectroscopic analysis with spectral autocorrelation," Opt. Express 12, 5487-5501 (2004). [CrossRef] [PubMed]
  9. R. N. Graf and A. Wax, "Nuclear morphology measurements using Fourier domain low coherence interferometry," Opt. Express 13, 4693-4698 (2005). [CrossRef]
  10. T. S. Troutman, J. K. Barton, and M. Romanowski, "Optical coherence tomography with plasmon resonant nanorods of gold," Opt. Lett. 32, 1438-1440 (2007). [CrossRef] [PubMed]
  11. J. Chen, F. Saeki, B. J. Wiley, Hu Cang, M. J. Cobb, Z.-Y. Li, L. Au, H. Zhang, M. B. Kimmey, X. Li, and Y. Xia, "Gold Nanocages: Bioconjugation and Their Potential Use as Optical Imaging Contrast Agents," Nano Lett. 5, 473-477 (2005). [CrossRef] [PubMed]
  12. J. K. Barton, N. J. Halas, J. L. West, and R. A. Drezek, "Nanoshells as an Optical Coherence Tomography Contrast Agent," Proc. SPIE, 5316, (2004). [CrossRef]
  13. A. M. Gobin, M. H. Lee, N. J. Halas, W. D. James, R. A. Drezek, and J. L. West, "Near-Infrared Resonant Nanoshells for Combined Optical Imaging and Photothermal Cancer Therapy," Nano Lett. 7, 1929-1934 (2007). [CrossRef] [PubMed]
  14. A. L. Oldenburg, M.N. Hansen, D. A. Zweifel, A. Wei, and S. A. Boppart, "Plasmon-resonant gold nanorods as low backscattering albedo contrast agents for optical coherence tomography," Opt. Express 14, 6724-6738 (2006). [CrossRef] [PubMed]
  15. W. Drexler, U. Morgner, F. X. Kartner, C. Pitris, S. A. Boppart, X. D. Li, E. P. Ippen, and J. G. Fujimoto, "In vivo ultrahigh-resolution optical coherence tomography," Opt. Lett. 24, 1221-1223 (1999). [CrossRef]
  16. A. Dubois, K. Grieve, G. Moneron, R. Lecaque, L. Vabre, and C. Boccara, "Ultrahigh-Resolution Full-Field Optical Coherence Tomography," Appl. Opt. 43, 2874-2883 (2004). [CrossRef] [PubMed]
  17. C. Xu, D. L. Marks, M N. Do, and S. A. Boppart, "Separation of absorption and scattering profiles in spectroscopic optical coherence tomography using a least-squares algorithm," Opt. Express 12, 4790-4803 (2004). [CrossRef] [PubMed]
  18. C. Xu, P. S. Carney, and S. A. Boppart, "Wavelength-dependent scattering in spectroscopic optical coherence tomography," Opt. Express 13, 5450-5462 (2005). [CrossRef] [PubMed]
  19. T. Dennis, S. D. D., A. Dienstfrey, G. Singh and P. Rice, "Analyzing quantitative light scattering spectra of phantoms measured with optical coherence tomography," J. Biomed. Opt. 13, 024004 (2008). [CrossRef] [PubMed]
  20. R. N. Graf, W. J. Browmn, and A. Wax, "Parallel frequency-domain optical coherence tomography scatter-mode imaging of the hamster cheek pouch using a thermal light source," Opt. Express 33, 1285-1287 (2008).
  21. Y. L. Xu "Electromagnetic scattering by an aggregate of spheresParallel frequency-domain optical coherence tomography scatter-mode imaging of the hamster cheek pouch using a thermal light source," Appl. Opt. 34, 4573-4588 (1995). [CrossRef] [PubMed]
  22. J. L. Hollmann, A. K. Dunn, and C. A. DiMarzio, "Computational microscopy in embryo imaging," Opt. Express 29, 2267-2269 (2004).
  23. Y. Liu, X. Li, Y. L. Kim, and V. Backman, "Elastic backscattering spectroscopic microscopy," Opt. Lett. 30, 2445-2447 (2005). [CrossRef] [PubMed]
  24. R. Cubeddu, A. Pifferi, P. Taroni, A. Torricelli, and G. Valentini, "A solid tissue phantom for photon migration studies," Phys. Med. Biol. 42, 1971-1979 (1997). [CrossRef] [PubMed]
  25. R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C. K. Hitzenberger, M. Sticker, and A. F. Fercher, "Spectral measurement of absorption by spectroscopic frequency-domain optical coherence tomography," Opt. Lett. 25, 820-822 (2000). [CrossRef]
  26. C. Xu, F. Kamalabadi, and S. A. Boppart, "Comparative performance analysis of time-frequency distributions for spectroscopic optical coherence tomography," Appl. Opt. 44, 1813-1822 (2005). [CrossRef] [PubMed]
  27. A. Wax, C. Yang, and J. A. Izatt, "Fourier-domain low-coherence interferometry for light-scattering spectroscopy," Opt. Lett. 28, 1230-1232 (2003). [CrossRef] [PubMed]

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