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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 10 — Oct. 2, 2009

Isotropic image in structured illumination microscopy patterned with a spatial light modulator

Bo-Jui Chang, Li-Jun Chou, Yun-Ching Chang, and Su-Yu Chiang  »View Author Affiliations


Optics Express, Vol. 17, Issue 17, pp. 14710-14721 (2009)
http://dx.doi.org/10.1364/OE.17.014710


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Abstract

We developed a structured illumination microscopy (SIM) system that uses a spatial light modulator (SLM) to generate interference illumination patterns at four orientations - 0°, 45°, 90°, and 135°, to reconstruct a high-resolution image. The use of a SLM for pattern alterations is rapid and precise, without mechanical calibration; moreover, our design of SLM patterns allows generating the four illumination patterns of high contrast and nearly equivalent periods to achieve a near isotropic enhancement in lateral resolution. We compare the conventional image of 100-nm beads with those reconstructed from two (0°+90° or 45°+135°) and four (0°+45°+90°+135°) pattern orientations to show the differences in resolution and image, with the support of simulations. The reconstructed images of 200-nm beads at various depths and fine structures of actin filaments near the edge of a HeLa cell are presented to demonstrate the intensity distributions in the axial direction and the prospective application to biological systems.

© 2009 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: April 30, 2009
Revised Manuscript: June 27, 2009
Manuscript Accepted: July 3, 2009
Published: August 5, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Bo-Jui Chang, Li-Jun Chou, Yun-Ching Chang, and Su-Yu Chiang, "Isotropic image in structured illumination microscopy patterned with a spatial light modulator," Opt. Express 17, 14710-14721 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-17-14710


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