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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 10 — Oct. 2, 2009

Ultra-fast multispectral optical imaging of cortical oxygenation, blood flow, and intracellular calcium dynamics

Matthew B. Bouchard, Brenda R. Chen, Sean A. Burgess, and Elizabeth M. C. Hillman  »View Author Affiliations


Optics Express, Vol. 17, Issue 18, pp. 15670-15678 (2009)
http://dx.doi.org/10.1364/OE.17.015670


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Abstract

Camera-based optical imaging of the exposed brain allows cortical hemodynamic responses to stimulation to be examined. Typical multispectral imaging systems utilize a camera and illumination at several wavelengths, allowing discrimination between changes in oxy- and deoxyhemoglobin concentration. However, most multispectral imaging systems utilize white light sources and mechanical filter wheels to multiplex illumination wavelengths, which are slow and difficult to synchronize at high frame rates. We present a new LED-based system capable of high-resolution multispectral imaging at frame rates exceeding 220 Hz. This improved performance enables simultaneous visualization of hemoglobin oxygenation dynamics within single vessels, changes in vessel diameters, blood flow dynamics from the motion of erythrocytes, and dynamically changing fluorescence.

© 2009 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 2, 2009
Revised Manuscript: August 3, 2009
Manuscript Accepted: August 9, 2009
Published: August 20, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Matthew B. Bouchard, Brenda R. Chen, Sean A. Burgess, and Elizabeth M. C. Hillman, "Ultra-fast multispectral optical imaging of cortical oxygenation, blood flow, and intracellular calcium dynamics," Opt. Express 17, 15670-15678 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-18-15670


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