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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 10 — Oct. 2, 2009

A STED microscope aligned by design

Dominik Wildanger, Johanna Bückers, Volker Westphal, Stefan W. Hell, and Lars Kastrup  »View Author Affiliations


Optics Express, Vol. 17, Issue 18, pp. 16100-16110 (2009)
http://dx.doi.org/10.1364/OE.17.016100


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Abstract

STED microscopes are commonly built using separate optical paths for the excitation and the STED beam. As a result, the beams must be co-aligned and can be subject to mechanical drift. Here, we present a single-path STED microscope whose beams are aligned by design and hence is insensitive to mechanical drift. The design of a phase plate is described which selectively modulates the STED beam but leaves the excitation beam unaffected. The performance of the single-beam setup is on par with previous dual-beam designs.

© 2009 OSA

OCIS Codes
(100.5090) Image processing : Phase-only filters
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Microscopy

History
Original Manuscript: July 28, 2009
Revised Manuscript: August 17, 2009
Manuscript Accepted: August 19, 2009
Published: August 26, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Dominik Wildanger, Johanna Bückers, Volker Westphal, Stefan W. Hell, and Lars Kastrup, "A STED microscope aligned by design," Opt. Express 17, 16100-16110 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-18-16100


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References

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