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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 11 — Oct. 21, 2009

Raman Microscopy based on Doubly-Resonant Four-Wave Mixing (DR-FWM)

Tyler Weeks, Sebastian Wachsmann-Hogiu, and Thomas Huser  »View Author Affiliations


Optics Express, Vol. 17, Issue 19, pp. 17044-17051 (2009)
http://dx.doi.org/10.1364/OE.17.017044


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Abstract

Doubly-resonant four-wave mixing (DR-FWM) is a nondegenerate four-wave mixing process in which four photons interact to coherently probe two distinct Raman resonances. We demonstrate DR-FWM microscopy as a label-free and nondestructive molecular imaging modality with high chemical specificity on the submicron scale by imaging alkyne-substituted oleic acid in both aqueous and lipid-rich environments. DR-FWM microscopy is contrasted to coherent anti-Stokes Raman scattering (CARS) microscopy and it is shown that the coherent addition of two simultaneously probed Raman resonances leads to a significant increase in signal without increasing the non-resonant background. Thus, this scheme enables the detection of weak Raman signals through amplification by a strong Raman resonance, potentially increasing the overall detection sensitivity beyond what has been demonstrated by either CARS or stimulated Raman scattering (SRS).

© 2009 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 5, 2009
Revised Manuscript: September 1, 2009
Manuscript Accepted: September 2, 2009
Published: September 9, 2009

Virtual Issues
Vol. 4, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Tyler Weeks, Sebastian Wachsmann-Hogiu, and Thomas Huser, "Raman Microscopy based on Doubly-Resonant Four-Wave Mixing (DR-FWM)," Opt. Express 17, 17044-17051 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-19-17044


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