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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 11 — Oct. 21, 2009

Optical tweezers with millikelvin precision of temperature-controlled objectives and base-pair resolution

Mohammed Mahamdeh and Erik Schäffer  »View Author Affiliations


Optics Express, Vol. 17, Issue 19, pp. 17190-17199 (2009)
http://dx.doi.org/10.1364/OE.17.017190


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Abstract

In optical tweezers, thermal drift is detrimental for high-resolution measurements. In particular, absorption of the trapping laser light by the microscope objective that focuses the beam leads to heating of the objective and subsequent drift. This entails long equilibration times which may limit sensitive biophysical assays. Here, we introduce an objective temperature feedback system for minimizing thermal drift. We measured that the infrared laser heated the objective by 0.7K per watt of laser power and that the laser focus moved relative to the sample by ≈1 nm/mK due to thermal expansion of the objective. The feedback stabilized the temperature of the trapping objective with millikelvin precision. This enhanced the long-term temperature stability and significantly reduced the settling time of the instrument to about 100 s after a temperature disturbance while preserving single DNA base-pair resolution of surface-coupled assays. Minimizing systematic temperature changes of the objective and concurrent drift is of interest for other high-resolution microscopy techniques. Furthermore, temperature control is often a desirable parameter in biophysical experiments.

© 2009 Optical Society of America

OCIS Codes
(000.2170) General : Equipment and techniques
(120.6780) Instrumentation, measurement, and metrology : Temperature
(140.6810) Lasers and laser optics : Thermal effects
(140.7010) Lasers and laser optics : Laser trapping
(170.0180) Medical optics and biotechnology : Microscopy
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

History
Original Manuscript: July 9, 2009
Revised Manuscript: August 14, 2009
Manuscript Accepted: August 17, 2009
Published: September 11, 2009

Virtual Issues
Vol. 4, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Mohammed Mahamdeh and Erik Schäffer, "Optical tweezers with millikelvin precision of temperature-controlled objectives and base-pair resolution," Opt. Express 17, 17190-17199 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-19-17190


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References

  1. W. Denk and W. W. Webb, "Optical measurement of picometer displacements of transparent microscopic objects," Appl. Opt. 29, 2382-2391 (1990). [CrossRef] [PubMed]
  2. E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, "Direct observation of base-pair stepping by RNA polymerase," Nature 438, 460-465 (2005). [CrossRef] [PubMed]
  3. W. J. Greenleaf, M. T. Woodside, and S. M. Block, "High-resolution, single-molecule measurements of biomolecular motion," Annu. Rev. Biophys. Biomol. Struct. 36, 171-190 (2007). [CrossRef] [PubMed]
  4. J. R. Moffitt, Y. R. Chemla, K. Aathavan, S. Grimes, P. J. Jardine, D. L. Anderson, and C. Bustamante, "Intersubunit coordination in a homomeric ring ATPase," Nature 457, 446-450 (2009). [CrossRef] [PubMed]
  5. A. R. Carter, G.M. King, T. A. Ulrich,W. Halsey, D. Alchenberger, and T. T. Perkins, "Stabilization of an optical microscope to 0.1 nm in three dimensions," Appl. Opt. 46, 421-427 (2007). [CrossRef] [PubMed]
  6. E. Schäffer, S. F. Nørrelykke, and J. Howard, "Surface forces and drag coefficients of microspheres near a plane surface measured with optical tweezers," Langmuir 23, 3654-3665 (2007). [CrossRef] [PubMed]
  7. M. D. Wang, H. Yin, R. Landick, J. Gelles, and S. M. Block, "Stretching DNA with optical tweezers," Biophys. J. 72, 1335-1346 (1997). [CrossRef] [PubMed]
  8. K. Neuman and S. Block, "Optical Trapping," Rev. Sci. Instrum. 75, 2787-2809 (2004). [CrossRef]
  9. K. Svoboda and S. M. Block, "Biological Applications of Optical Forces," Annu. Rev. Biophys. Biomol. Struct. 23, 247-285 (1994). [CrossRef] [PubMed]
  10. H. B. Mao, J. R. Arias-Gonzalez, S. B. Smith, I. Tinoco, and C. Bustamante, "Temperature control methods in a laser tweezers system," Biophys. J. 89, 1308-1316 (2005). [CrossRef] [PubMed]
  11. E.Fällman and O. Axner, "Design for fully steerable dual-trap optical tweezers," Appl. Opt. 36, 2107-2113 (1997). [CrossRef] [PubMed]
  12. V. Bormuth, J. Howard, and E. Schäffer, "LED illumination for video-enhanced DIC imaging of single microtubules," J. Microsc. 226, 1-5 (2007). [CrossRef] [PubMed]
  13. A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, "Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light," Microsc. Res. Tech. 44, 378-386 (1999). [CrossRef] [PubMed]
  14. S. F. Tolić-Nørrelykke, E. Schäffer, J. Howard, F. S. Pavone, F. Jülicher, and H. Flyvbjerg, "Calibration of optical tweezers with positional detection in the back focal plane," Rev. Sci. Instrum. 77, 103,101 (2006).
  15. G. M. Gibson, J. Leach, S. Keen, A. J. Wright, and M. J. Padgett, "Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy," Opt. Express 16, 14,561-14,570 (2008). [CrossRef]
  16. A. R. Carter, Y. Seol, and T. T. Perkins, "Precision Surface-Coupled Optical-Trapping Assay with One-Basepair Resolution," Biophys. J. 96, 2926-2934 (2009). [CrossRef] [PubMed]
  17. S. W. Hell and J. Wichmann, "Breaking the diffraction resolution limit by stimulated-emission—stimulatedemission- depletion fluorescence microscopy," Opt. Lett. 19, 780-782 (1994). [CrossRef] [PubMed]

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