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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 12 — Nov. 10, 2009

Assessing light scattering of intracellular organelles in single intact living cells

Maxim Kalashnikov, Wonshik Choi, Chung-Chieh Yu, Yongjin Sung, Ramachandra R. Dasari, Kamran Badizadegan, and Michael S. Feld  »View Author Affiliations

Optics Express, Vol. 17, Issue 22, pp. 19674-19681 (2009)

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This report presents a model-independent method of assessing contributions to the light scattering from individual organelles in single intact cells. We first measure the 3D index map of a living cell, and then modify the map in such a way so as to eliminate contrast due to a particular intracellular organelle. By calculating and comparing the light scattering distributions calculated from the original and modified index maps using the Rytov approximation, we extract the light scattering contribution from the particular organelle of interest. The relative contributions of the nucleus and nucleolus to the scattering of the entire cell are thus determined, and the applicability of the homogeneous spherical model to non-spherical and heterogeneous organelles in forward scattering is evaluated.

© 2009 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.4020) Scattering : Mie theory
(290.5820) Scattering : Scattering measurements
(290.2558) Scattering : Forward scattering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 13, 2009
Revised Manuscript: September 27, 2009
Manuscript Accepted: September 28, 2009
Published: October 15, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Maxim Kalashnikov, Wonshik Choi, Chung-Chieh Yu, Yongjin Sung, Ramachandra R. Dasari, Kamran Badizadegan, and Michael S. Feld, "Assessing light scattering of intracellular organelles in single intact living cells," Opt. Express 17, 19674-19681 (2009)

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  1. V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, “Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ,” IEEE J. Sel. Top. Quantum Electron. 5(4), 1019–1026 (1999). [CrossRef]
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