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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 12 — Nov. 10, 2009

Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF Microscopy

Gaëlle Recher, Denis Rouède, Patrick Richard, Antoine Simon, Jean-Jaques Bellanger, and François Tiaho  »View Author Affiliations


Optics Express, Vol. 17, Issue 22, pp. 19763-19777 (2009)
http://dx.doi.org/10.1364/OE.17.019763


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Abstract

We have extensively characterized the sarcomeric SHG signal as a function of animal species (rat versus xenopus), age (adult versus larval) and tissue preparation (fixed or fresh) and we found that the main feature of this signal is a single peak per mature sarcomere (about 85% of all sarcomeres). The remaining (15%) was found to be either double peak per mature sarcomere or mini sarcomeres (half of a sarcomere) using α-actinin immuno detection of the Z-band. The mini sarcomeres are often found in region of pitchfork-like SHG pattern. We suggest that double peak SHG pattern could indicate regions of sarcomeric proteolysis whereas pitchfork-like SHG pattern could reveal sarcomeric assembly.

© 2009 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 23, 2009
Revised Manuscript: September 25, 2009
Manuscript Accepted: October 7, 2009
Published: October 16, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Gaëlle Recher, Denis Rouède, Patrick Richard, Antoine Simon, Jean-Jacques Bellanger, and François Tiaho, "Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF Microscopy," Opt. Express 17, 19763-19777 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-22-19763


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