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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 13 — Dec. 2, 2009

Optics InfoBase > Virtual Journal for Biomedical Optics > Volume 4 > Issue 13 > Page 20700

All-fiber CARS microscopy of live cells

Adrian F. Pegoraro, Andrew Ridsdale, Douglas J. Moffatt, John P. Pezacki, Brian K. Thomas, Libin Fu, Liang Dong, Martin E. Fermann, and Albert Stolow  »View Author Affiliations


Optics Express, Vol. 17, Issue 23, pp. 20700-20706 (2009)
http://dx.doi.org/10.1364/OE.17.020700


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Abstract

Using an all-fiber laser system consisting of a femtosecond Er/Yb fiber oscillator as the pump and an ultra-highly nonlinear fiber for Stokes generation, we demonstrate multimodal (TPF+SHG+CARS) nonlinear optical microscopy of both tissue samples and live cells. Multimodal imaging was successfully performed with pixel dwell times as short as 4 µs at low laser powers (<40 mW total).

© 2009 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 16, 2009
Revised Manuscript: October 19, 2009
Manuscript Accepted: October 20, 2009
Published: October 27, 2009

Virtual Issues
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Adrian F. Pegoraro, Andrew Ridsdale, Douglas J. Moffatt, John P. Pezacki, Brian K. Thomas, Libin Fu, Liang Dong, Martin E. Fermann, and Albert Stolow, "All-fiber CARS microscopy of live cells," Opt. Express 17, 20700-20706 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-23-20700


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