OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

A multimodal platform for nonlinear optical microscopy and microspectroscopy

Hongtao Chen, Haifeng Wang, Mikhail N. Slipchenko, YooKyung Jung, Yunzhou Shi, Jiabin Zhu, Kimberly K. Buhman, and Ji-Xin Cheng  »View Author Affiliations


Optics Express, Vol. 17, Issue 3, pp. 1282-1290 (2009)
http://dx.doi.org/10.1364/OE.17.001282


View Full Text Article

Enhanced HTML    Acrobat PDF (666 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.

© 2009 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Microscopy

History
Original Manuscript: November 12, 2008
Revised Manuscript: December 27, 2008
Manuscript Accepted: January 2, 2009
Published: January 21, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Hongtao Chen, Haifeng Wang, Mikhail N. Slipchenko, YooKyung Jung, Yunzhou Shi, Jiabin Zhu, Kimberly K. Buhman, and Ji-Xin Cheng, "A multimodal platform for nonlinear optical microscopy and microspectroscopy," Opt. Express 17, 1282-1290 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-3-1282


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990). [CrossRef] [PubMed]
  2. P. J. Campagnola and L. M. Loew, "Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms," Nat. Biotech. 21, 1356-1360 (2003). [CrossRef]
  3. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, "Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation," Proc. Natl. Acad. Sci. U. S. A. 100, 7075-7080 (2003). [CrossRef] [PubMed]
  4. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, "Nonlinear scanning laser microscopy by third harmonic generation," Appl. Phys. Lett. 70, 922-924 (1997). [CrossRef]
  5. J. Squier, M. Muller, G. Brakenhoff, and K. R. Wilson, "Third harmonic generation microscopy," Opt. Express 3, 315-324 (1998). [CrossRef] [PubMed]
  6. D. Debarre, W. Supatto, A.-M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M.-C. Schanne-Klein, and E. Beaurepaire, "Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy," Nat. Meth. 3, 47-53 (2006). [CrossRef]
  7. J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "An epi-detected coherent anti-Stokes Raman scattering (E-CARS) microscope with high spectral resolution and high sensitivity," J. Phys. Chem. B 105, 1277-1280 (2001). [CrossRef]
  8. F. Ganikhanov, S. Carrasco, X. S. Xie, M. Katz, W. Seitz, and D. Kopf, "Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy," Opt. Lett. 31, 1292-1294 (2006). [CrossRef] [PubMed]
  9. M. Hashimoto, T. Araki, and S. Kawata, "Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration," Opt. Lett. 25, 1768-1770 (2000). [CrossRef]
  10. Y. Fu, H. Wang, R. Shi, and J.-X. Cheng, "Second harmonic and sum frequency generation imaging of fibrous astroglial filaments in ex vivo spinal tissues," Biophys. J. 92, 3251-3259 (2007). [CrossRef] [PubMed]
  11. H.-W. Wang, T. T. Le, and J.-X. Cheng, "Label-free imaging of arterial cells and extracellular matrix using a multimodal CARS microscope," Opt. Commun. 281, 1813-1822 (2008). [CrossRef]
  12. J. X. Cheng and X. S. Xie, "Coherent anti-Stokes Raman scattering microscopy: instrumentation, theory, and applications," J. Phys. Chem. B 108, 827-840 (2004). [CrossRef]
  13. S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy," J. Biomed. Opt. 11, 020501-020503 (2006). [CrossRef] [PubMed]
  14. Y. Fu, H. Wang, T. B. Huff, R. Shi, and J.-X. Cheng, "Coherent anti-stokes Raman scattering imaging of myelin degradation reveals a calcium-dependent pathway in lyso-PtdCho-induced demyelination," J. Neurosci. Res. 85, 2870-2881 (2007). [CrossRef] [PubMed]
  15. J. Moger, B. D. Johnston, and C. R. Tyler, "Imaging metal oxide nanoparticles in biological structures with CARS microscopy," Opt. Express 16, 3408-3419 (2008). [CrossRef] [PubMed]
  16. X. Nan, J.-X. Cheng, and X. S. Xie, "Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy," J. Lipid Res. 44, 2202-2208 (2003). [CrossRef] [PubMed]
  17. L. Li, H. Wang, and J.-X. Cheng, "Quantitative Coherent Anti-Stokes Raman Scattering Imaging of Lipid Distribution in Coexisting Domains," Biophys. J. 89, 3480-3490 (2005). [CrossRef] [PubMed]
  18. T. Hellerer, C. Axäng, C. Brackmann, P. Hillertz, M. Pilon, and A. Enejder, "Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy," Proc. Natl. Acad. Sci. U. S. A. 104, 14658-14663 (2007). [CrossRef] [PubMed]
  19. J.-X. Cheng, and X. S. Xie, "Green's function formulation for third-harmonic generation microscopy," J. Opt. Soc. Am. B 19, 1604-1610 (2002). [CrossRef]
  20. S. Huang, A. A. Heikal, and W. W. Webb, "Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein," Biophys. J. 82, 2811-2825 (2002). [CrossRef] [PubMed]
  21. M. D. Duncan, J. Reintjes, and T. J. Manuccia, "Scanning coherent anti-Stokes Raman microscope," Opt. Lett. 7, 350-352 (1982). [CrossRef] [PubMed]
  22. A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering," Phys. Rev. Lett. 82, 4142 (1999). [CrossRef]
  23. E. O. Potma, D. J. Jones, J.-X. Cheng, X. S. Xie, and J. Ye, "High-sensitivity coherent anti-Stokes Raman scattering microscopy with two tightly synchronized picosecond lasers," Opt. Lett. 27, 1168-1170 (2002). [CrossRef]
  24. C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, "Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy," Proc. Natl. Acad. Sci. U. S. A. 102, 16807-16812 (2005). [CrossRef] [PubMed]
  25. N. Dudovich, D. Oron, and Y. Silberberg, "Single-pulse coherently controlled nonlinear Raman spectroscopy and microscopy," Nature 418, 512-514 (2002). [CrossRef] [PubMed]
  26. S.-H. Lim, A. G. Caster, and S. R. Leone, "Single-pulse phase-control interferometric coherent anti-Stokes Raman scattering spectroscopy," Phys. Rev. A 72, 041803 (2005). [CrossRef]
  27. J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-Stokes Raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106, 8493-8498 (2002). [CrossRef]
  28. M. Muller, and J. M. Schins, "Imaging the thermodynamic state of lipid membranes with multiplex CARS microscopy," J. Phys. Chem. B 106, 3715-3723 (2002). [CrossRef]
  29. H. A. Rinia, K. N. J. Burger, M. Bonn, and M. Muller, "Quantitative label-free imaging of lipid composition and packing of individual cellular lipid droplets using multiplex CARS microscopy," Biophys. J. 95, 4908-4914 (2008). [CrossRef] [PubMed]
  30. H. N. Paulsen, K. M. Hilligse, J. Thøgersen, S. R. Keiding, and J. J. Larsen, "Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source," Opt. Lett. 28, 1123-1125 (2003). [CrossRef] [PubMed]
  31. T. W. Kee, and M. T. Cicerone, "Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy," Opt. Lett. 29, 2701-2703 (2004). [CrossRef] [PubMed]
  32. H. Kano, and H.-o. Hamaguchi, "Ultrabroadband (> 2500 cm-1) multiplex coherent anti-Stokes Raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber," Appl. Phys. Lett. 86, 121113-121113 (2005). [CrossRef]
  33. G. I. Petrov, and V. Yakovlev, "Enhancing red-shifted white-light continuum generation in optical fibers for applications in nonlinear Raman microscopy," Opt. Express 13, 1299-1306 (2005). [CrossRef] [PubMed]
  34. T. Hellerer, A. M. K. Enejder, and A. Zumbusch, "Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses," Appl. Phys. Lett. 85, 25-27 (2004). [CrossRef]
  35. Y. Fu, H. Wang, R. Shi, and J.-X. Cheng, "Characterization of photodamage in coherent anti-Stokes Raman scattering microscopy," Opt. Express 14, 3942-3951 (2006). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited