Diffraction Phase Cytometry: blood on a CD-ROM
Optics Express, Vol. 17, Issue 4, pp. 2579-2585 (2009)
http://dx.doi.org/10.1364/OE.17.002579
Enhanced HTML 
Acrobat PDF (400 KB)
Abstract
We demonstrate Diffraction Phase Cytometry (DPC) as a single shot, full-field, high throughput quantitative phase imaging modality, dedicated to analyzing whole blood smears. Utilizing a commercial CD as a sample substrate, along with dynamic spatial filtering via a liquid crystal spatial light modulator, we have developed a compact instrument capable of making quantitative, physiologically relevant measurements. To illustrate the ability of the system to function as a highly sensitive cytometer we imaged a large number (N=1,537) of live human erythrocytes in whole blood without preparation. We retrieved a comprehensive set of geometrical parameters including cell volume and surface area, which are not directly available using existing cytometers. Furthermore, we retrieved the minimum cylindrical diameter, through which red blood cells can pass, and deliver oxygen. These initial results prove the concept for an inexpensive lab-on-a-chip blood screening device.
© 2009 Optical Society of America
OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: December 17, 2008
Revised Manuscript: January 30, 2009
Manuscript Accepted: February 1, 2009
Published: February 6, 2009
Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics
Citation
Mustafa Mir, Zhuo Wang, Krishnarao Tangella, and Gabriel Popescu, "Diffraction Phase Cytometry: blood on a CD-ROM," Opt. Express 17, 2579-2585 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-4-2579
Sort: Year | Journal | Reset
References
- F. Brochard and J. F. Lennon, "Frequency spectrum of the flicker phenomenon in erythrocytes," J. Physique 36, 1035-1047 (1975). [CrossRef]
- N. Gov, A. G. Zilman and S. Safran, "Cytoskeleton confinement and tension of red blood cell membranes," Phys. Rev. Lett. 90, 228101 (2003). [CrossRef] [PubMed]
- P. B. Canham and A. C. Burton, "Distribution of Size and Shape in Populations of Normal Human Red Cells," Circ. Res. 22, 405-422 (1968). [PubMed]
- K. G. E. a H. J. Meiselman, "Effects of Pressure on Red Blood Cell Geometry during Micropipette Aspiration," Cytometry 23, 22-27 (1996). [CrossRef]
- W. Groner, N. Mohandas, and M. Bessis, "New Optical Technique for Measuring Erythrocyte Deformability with Ektacytometer," Clin. Chem. 26, (1980). [PubMed]
- D. J. Stephens and V. J. Allan, "Light Microscopy Techniques for Live Cell Imaging," Science 300, 82-86 (2003). [CrossRef] [PubMed]
- G. Popescu, Methods in Nano Cell Biology, 90, 87, P. J. Bhanu, ed., (Elsevier, 2008).
- G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, "Diffraction phase microscopy for quantifying cell structure and dynamics," Opt. Lett. 31, 775-777 (2006). [CrossRef] [PubMed]
- Z. Wang, L. J. Millet, M. U. Gillette, and G. Popescu, "Jones phase microscopy of transparent and anisotropic samples," Opt. Lett. 33, 1270-1272 (2008). [CrossRef] [PubMed]
- F. Charriere et al., "Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy," Opt. Lett. 32, 2456-2458 (2007). [CrossRef] [PubMed]
- J. B. Bain, Blood Cells, A Practical Guide (Blackwell Science, 2002).
- Y. L. Jin, J. Y. Chen, L. Xu, and P. N. Wang, "Refractive index measurement for biomaterial samples by total internal reflection," Phys. Med. Biol. 51, 371-379 (2006). [CrossRef]
- M. Hammer, D. Schweitzer, B. Michel, E. Thamm, and A. Kolb, "Single scattering by red blood cells," Appl. Opt. 37, 7410-7418 (1998). [CrossRef]
- R. C. Gonzalez, R. E. Woods, and S. L. Eddins, Digital Image Processing Using MATLAB (Pearson Prentice Hall, NJ, 2004).
- B. Rappaz et al., "Comparative Study of Human Ertythrocytes by Digital Holographic Microscopy," Confocal Microscopy, and Impedance Volume Analyzer, Cyto. A. 73A, 895-903 (2008).
- R. P. Rand and A. C. Burton, "Area and volume changes in hemolysis of single erythrocytes," J. Cell. Comp. Physiol. 61, 245-253 (1963). [CrossRef] [PubMed]
Cited By |
 Alert me when this paper is cited |
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.
Related Journal Articles 
- Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy (OL)
- Adaptive Scanning Optical Microscope (ASOM): A multidisciplinary optical microscope design for large field of view and high resolution imaging (OE)
- Quantitative phase imaging of live cells using fast Fourier phase microscopy (AO)
- Digital holography of total internal reflection (OE)
- Single-shot slightly-off-axis interferometry based Hilbert phase microscopy of red blood cells (BOE)
Related Conference Papers
- Optical coherence tomographic imaging of in vivo cellular dynamics
- Drug response measurements using optical forces
- Advances in development of a quadrature tomographic microscope
- Linear, Spatio-Temporal Characterization of UV Microscope Objectives for Nonlinear Imaging and Spectroscopy
- Linear, Spatio-Temporal Characterization of UV Microscope Objectives for Nonlinear Imaging and Spectroscopy
« Previous Article | Next Article »
OSA is a member of 