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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

Analog mean-delay method for high-speed fluorescence lifetime measurement

Sucbei Moon, Youngjae Won, and Dug Young Kim  »View Author Affiliations


Optics Express, Vol. 17, Issue 4, pp. 2834-2849 (2009)
http://dx.doi.org/10.1364/OE.17.002834


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Abstract

We present a new high-speed lifetime measurement scheme of analog mean-delay (AMD) method which is suitable for studying dynamical time-resolved spectroscopy and high-speed fluorescence lifetime imaging microscopy (FLIM). In our lifetime measurement method, the time-domain intensity signal of a fluorescence decay is acquired as an analog waveform. And the lifetime information is extracted from the mean temporal delay of the acquired signal. Since this method does not rely on the single-photon counting technique, the signals of multiple fluorescence photons can be acquired simultaneously. The measurement speed can be increased easily by raising the fluorescence intensity without a photon-rate limit. We have investigated various characteristics of our method in lifetime accuracy and precision as well as measurement speed. It has been found that our method can provide excellent measurement performances in various aspects. We have demonstrated a high-speed measurement with a high photon detection rate of ~108 photons per second with a nearly shot noise-limited photon economy. A fluorescence lifetime of 3.2 ns was accurately determined with a standard deviation of 3% from the data acquired within 17.8 μs at a rate of 56,300 lifetime determinations per second.

© 2009 Optical Society of America

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Spectroscopy

History
Original Manuscript: December 18, 2008
Revised Manuscript: February 9, 2009
Manuscript Accepted: February 10, 2009
Published: February 11, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Sucbei Moon, Youngjae Won, and Dug Young Kim, "Analog mean-delay method for high-speed fluorescence lifetime measurement," Opt. Express 17, 2834-2849 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-4-2834


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