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Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

Limits for reduction of effective focal volume in multiple-beam light microscopy

Anton Arkhipov and Klaus Schulten  »View Author Affiliations


Optics Express, Vol. 17, Issue 4, pp. 2861-2870 (2009)
http://dx.doi.org/10.1364/OE.17.002861


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Abstract

Employing interference patterns for illumination has been shown to reduce the focal volume in fluorescence microscopy. For example, the 4Pi technique employs two interfering laser beams and significantly decreases the focal volume, as compared to conventional microscopy. We study theoretically the effect of using multiple interfering laser beams on the focal volume. In realistic setups with three or four beams, the focal volume is about half of that from the 4Pi case. This improvement reaches a limit quickly as more beams are added, and for the idealized case of an infinite number of beams the focal volume is rather close to the three- or four-beam cases. Thus, our study suggests a limit for the possible reduction of the focal volume in a purely optical far-field setup.

© 2009 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Microscopy

History
Original Manuscript: December 22, 2008
Revised Manuscript: February 4, 2009
Manuscript Accepted: February 7, 2009
Published: February 11, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Anton Arkhipov and Klaus Schulten, "Limits for reduction of effective focal volume in multiple-beam light microscopy," Opt. Express 17, 2861-2870 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-4-2861


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