OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Sensitive DNA biosensor based on a long-period grating formed on the side-polished fiber surface

Hyun Soo Jang, Kwang No Park, Jun P. Kim, Sang Jun Sim, Oh J. Kwon, Young-Geun Han, and Kyung Shik Lee  »View Author Affiliations

Optics Express, Vol. 17, Issue 5, pp. 3855-3860 (2009)

View Full Text Article

Enhanced HTML    Acrobat PDF (684 KB) Open Access

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We demonstrate a sensitive DNA biosensor based on a long period grating (LPG) formed by a photolithograph process on the surface of a side-polished fiber. The biomolecules of the biosensor were immobilized on the silica surface between LPG patterns. The resonance wavelength was red-shifted after the binding of the poly-L-lysine, probe ssDNA and target ssDNA to the sensor surface. The overall wavelength shift after the successful DNA hybridization was 1.82 nm. The proposed LPG-based DNA biosensor is ~2.5 times more sensitive than the previously reported fiber grating-based DNA biosensors.

© 2009 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 29, 2009
Revised Manuscript: February 24, 2009
Manuscript Accepted: February 24, 2009
Published: February 26, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Hyun Soo Jang, Kwang No Park, Jun P. Kim, Sang Jun Sim, Oh J. Kwon, Young-Geun Han, and Kyung Shik Lee, "Sensitive DNA biosensor based on a long-period grating formed on the side-polished fiber surface," Opt. Express 17, 3855-3860 (2009)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. P. T. Charles, G. J. Vora, J. D. Andreadis, A. J. Fortney, C. E. Meador, C. S. Dulcey, and D. A. Stenger, "Fabrication and Surface Characterization of DNA Microarrays Using Amine- and Thiol-Terminated Oligonucleotide Probes," Langmuir 19, 1586-1591 (2003). [CrossRef]
  2. J. Homola, S. S. Yee, and G. Gauglitz, "Surface plasmon resonance sensors: review," Sens. Actuators B 54, 3-15 (1999). [CrossRef]
  3. T. M. Chinowskya, J. G. Quinn, D. U. Bartholomew, R. Kaiser, and J. L. Elkind, "Performance of the Spreeta 2000 integrated surface plasmon resonance affinity sensor," Sens. Actuators B 91, 266-274 (2003). [CrossRef]
  4. M. Mehrvar, C. Bis, J. M. Scharer, M. Moo-Young, and J. H. Luong, "Fiber optic biosensors-trends and advances," Anal. Sci. 16, 677-692 (2000). [CrossRef]
  5. J. F. Masson, M. Barnhart, T. M. Battaglia, G. E. Morris, R. A. Nieman, P. J. Young, C. L. Lorson, and K. S. Booksh, "Monitoring of recombinant survival motor neuron protein using fiber-optic surface plasmon resonance," Analyst 129, 855-859 (2004). [CrossRef] [PubMed]
  6. S. Maguis, G. Laffont, P. Ferdinand, B. Carbonnier, K. Kham, T. Mekhalif, and M. Millot, "Biofunctionalized tilted Fiber Bragg Gratings for label-free immunosensing," Opt. Express 16, 19049-19062 (2008). [CrossRef]
  7. A. N. Chryssis, S. S. Saini, S. M. Lee, H. Yi, W. E. Bentley, and M. Dagenais, "Detecting Hybridization of DNA by highly sensitive evanescent field etched core fiber bragg grating sensors," IEEE J. Sel. Top. Quantum. Electron. 11, 864-872 (2005). [CrossRef]
  8. J. L. Tang, S. F. Cheng, W. T. Hsu, T. Y. Chiang, and L. K. Chau, "Fiber optic biochemical sensing with a colloidal gold modified long period fiber grating," Sens. Actuators B 119, 105-109 (2006). [CrossRef]
  9. J. Yang, P. Sandhu, W. Liang, C. Xu, and Y. Li, "Label free fiber optic biosensors with enhanced sensitivity," IEEE J. Sel. Top. Quantum. Electron. 13, 1691-1696 (2007). [CrossRef]
  10. X. Wei, T. Wei, H. Xiao, and Y. S. Lin, "Nano structured Pd-long period fiber gratings integrated optical sensor for hydrogen detection," Sens. Actuators B 134, 687-693 (2008). [CrossRef]
  11. K. S. Lee and T. Erdogan, "Fiber mode coupling in transmissive and reflective tilted fiber gratings," Appl. Opt. 39, 1394-1404 (2000). [CrossRef]
  12. H. S. Jang, K. N. Park, and K. S. Lee, "Characterization of tunable photonic crystal fiber directional couplers," Appl. Opt. 46, 3688-3693 (2007). [CrossRef] [PubMed]
  13. K. S. Chiang, Y. Q. Liu, M. N. Ng, and X. Y. Dong, "Analysis of etched long-period fibre grating and its response to external refractive index," Electron. Lett. 36, 966-967 (2000). [CrossRef]
  14. I. M. Ishaq, A. Quintela, S. W. James, G. J. Ashwell, J. M. Lopez-Higuera, and R. P. Tatam, "Modification of the refractive index response of long period gratings using thin film overlays," Sens. Actuators B 107, 738-741 (2005). [CrossRef]
  15. J. Yang, L. Yang, C. Q. Xu, C. Xu, W. Huang, and Y. Li, "Long-period grating refractive index sensor with a modified cladding structure for large operational range and high sensitivity," Appl. Opt. 45, 6142-6147 (2006). [CrossRef] [PubMed]
  16. A. M. Vengsarkar, P. J. Lemaire, J. B. Judkins, V. Bhatia, T. Erdogan, and J. E. Sipe, "Long-period fiber gratings as band-rejection filters," J. Lightwave Technol. 14, 58-65 (1996). [CrossRef]
  17. A. Iadicicco, A. Cusano, A. Cutolo, R. Bernini, and M. Giordano, "Thinned fiber bragg gratings as high sensitivity refractive index sensor," IEEE Photon. Technol. Lett. 16, 1149-1151 (2004). [CrossRef]
  18. P. D. Sawant and D. V. Nicolau, "Hierarchy of DNA immobilization and hybridization on poly-L-lysine:an atomic force microscopy study," Smart Mater. Struct. 15, S99-S103 (2006). [CrossRef]
  19. C. E. Jordan, B. L. Frey, S. Kornguth, and R. M. Corn, "Characterization of poly-L-lysine adsorption onto alkanethiol modified gold surface with polarization modulation fourier transform infrared spectroscopy and surface Plasmon resonance measurements," Langmuir 10, 3642-3648 (1994). [CrossRef]
  20. X. Chen, L. Zhang, K. Zhou, E. Davies, K. Sugden, I. Bennion, M. Hughes, and A. Hine, "Real time detection of DNA interactions with long period fiber grating based biosensor," Opt. Lett. 32, 2541-2543 (2007). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited