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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Anterior segment imaging with Spectral OCT system using a high-speed CMOS camera

Ireneusz Grulkowski, Michalina Gora, Maciej Szkulmowski, Iwona Gorczynska, Daniel Szlag, Susana Marcos, Andrzej Kowalczyk, and Maciej Wojtkowski  »View Author Affiliations


Optics Express, Vol. 17, Issue 6, pp. 4842-4858 (2009)
http://dx.doi.org/10.1364/OE.17.004842


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Abstract

We describe a new ultrahigh speed Spectral OCT instrument making use of a CMOS camera and demonstrate high quality in vivo imaging of the anterior segment of the human eye. The high flexibility of the designed imaging system allows a wide range of imaging protocols. Two- and three-dimensional high quality OCT images of the cornea, the anterior chamber and the crystalline lens are presented. A high acquisition rate, up to 135,000 A-scans/second enables three-dimensional reconstruction of the anterior segment during lenticular accommodation, blinking and pupillary reaction to light stimulus. We demonstrate OCT tomographic real time imaging of the lens dynamics during accommodation and high quality OCT cross-sectional images of the entire anterior segment of the eye from the cornea up to posterior part of the crystalline lens.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 12, 2009
Revised Manuscript: March 6, 2009
Manuscript Accepted: March 8, 2009
Published: March 12, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Ireneusz Grulkowski, Michalina Gora, Maciej Szkulmowski, Iwona Gorczynska, Daniel Szlag, Susana Marcos, Andrzej Kowalczyk, and Maciej Wojtkowski, "Anterior segment imaging with Spectral OCT system using a high-speed CMOS camera," Opt. Express 17, 4842-4858 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-6-4842


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References

  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991). [CrossRef] [PubMed]
  2. A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, "Measurement of intraocular distances by backscattering spectral interferometry," Opt. Commun. 117, 43-48 (1995). [CrossRef]
  3. A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, "Optical coherence tomography: a review of clinical development from bench to bedside," J. Biomed. Opt. 12, 051403 (2007). [CrossRef] [PubMed]
  4. W. Drexler, "Ultrahigh-resolution optical coherence tomography," J. Biomed. Opt. 9, 47-74 (2004). [CrossRef] [PubMed]
  5. Z. Chen, T. E. Milner, D. Dave, and J. S. Nelson, "Optical Doppler tomographic imaging of fluid flow velocity in highly scattering media," Opt. Lett. 22, 64-66 (1997). [CrossRef] [PubMed]
  6. Z. P. Chen, T. E. Milner, S. Srinivas, X. J. Wang, A. Malekafzali, M. J. C. vanGemert, and J. S. Nelson, "Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography," Opt. Lett. 22, 1119-1121 (1997). [CrossRef] [PubMed]
  7. M. Szkulmowski, A. Szkulmowska, T. Bajraszewski, A. Kowalczyk, and M. Wojtkowski, "Flow velocity estimation using joint Spectral and Time domain Optical Coherence Tomography," Opt. Express 16, 6008-6025 (2008). [CrossRef] [PubMed]
  8. U. Morgner, W. Drexler, F. X. Kartner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, "Spectroscopic optical coherence tomography," Opt. Lett. 25, 111-113 (2000). [CrossRef]
  9. B. E. Applegate, C. Yang, and J. Izatt, "Theoretical comparison of the sensitivity of molecular contrast optical coherence tomography techniques," Opt. Express 13, 8146-8163 (2005). [CrossRef] [PubMed]
  10. J. A. Izatt, M. D. Kulkami, S. Yazdanfar, J. K. Barton, and A. J. Welch, "In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography," Opt. Lett. 22, 1439-1441 (1997). [CrossRef]
  11. J. F. De Boer, T. E. Milner, M. J. C. van Gemert, and J. S. Nelson, "Two-dimensional birefringence imaging in biological tissue by polarization-sensitive optical coherence tomography," Opt. Lett. 22, 934-936 (1997). [CrossRef] [PubMed]
  12. R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C. K. Hitzenberger, M. Sticker, and A. F. Fercher, "Spectral measurement of absorption by spectroscopic frequency-domain optical coherence tomography," Opt. Lett. 25, 820-822 (2000). [CrossRef]
  13. G. Hausler and M. W. Lindner, ""Coherence Radar" and "Spectral Radar" - New Tools for Dermatological Imaging," J. Biomed. Opt. 3, 21-31 (1998). [CrossRef]
  14. M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, and A. F. Fercher, "In vivo human retinal imaging by Fourier domain optical coherence tomography," J. Biomed. Opt. 7, 457-463 (2002). [CrossRef] [PubMed]
  15. S. H. Yun, G. J. Tearney, J. F. de Boer, N. Iftimia, and B. E. Bouma, "High-speed optical frequency-domain imaging," Opt. Express 11, 2953-2963 (2003). [CrossRef] [PubMed]
  16. M. Wojtkowski, V. J. Srinivasan, T. H. Ko, J. G. Fujimoto, A. Kowalczyk, and J. S. Duker, "Ultrahigh-resolution high-speed Fourier domain optical coherence tomography and methods for dispersion compensation," Opt. Express 12, 2404-2422 (2004). [CrossRef] [PubMed]
  17. M. Wojtkowski, T. Bajraszewski, P. Targowski, and A. Kowalczyk, "Real-time in vivo imaging by high-speed spectral optical coherence tomography," Opt. Lett. 28, 1745-1747 (2003). [CrossRef] [PubMed]
  18. R. Huber, M. Wojtkowski, and J. G. Fujimoto, "Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography," Opt. Express 14, 3225-3237 (2006). [CrossRef] [PubMed]
  19. R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, "Performance of Fourier domain vs. time domain optical coherence tomography," Opt. Express 11, 889-894 (2003). [CrossRef] [PubMed]
  20. J. F. de Boer, B. Cense, B. H. Park, M. C. Pierce, G. J. Tearney, and B. E. Bouma, "Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography," Opt. Lett. 28, 2067-2069 (2003). [CrossRef] [PubMed]
  21. M. A. Choma, M. V. Sarunic, C. H. Yang, and J. A. Izatt, "Sensitivity advantage of swept source and Fourier domain optical coherence tomography," Opt. Express 11, 2183-2189 (2003). [CrossRef] [PubMed]
  22. T. Dada, R. Sihota, R. Gadia, A. Aggarwal, S. Mandal, and V. Gupta, "Comparison of anterior segment optical coherence tomography and ultrasound biomicroscopy for assessment of the anterior segment," J. Cataract Refract. Surg. 33, 837-840 (2007). [CrossRef] [PubMed]
  23. A. Konstantopoulos, P. Hossain, and D. F. Anderson, "Recent advances in ophthalmic anterior segment imaging: a new era for ophthalmic diagnosis?," Br. J. Ophthalmol. 91, 551-557 (2007). [CrossRef] [PubMed]
  24. W. Nolan, "Anterior segment imaging: ultrasound biomicroscopy and anterior segment optical coherence tomography," Curr. Opin. Ophthalmol. 19, 115-121 (2008). [CrossRef] [PubMed]
  25. W. J. Dupps, "Anterior segment imaging: New milestones, new challenges," J. Cataract. Refract. Surg. 32, 1779-1783 (2006). [CrossRef] [PubMed]
  26. P. Rosales, M. Dubbelman, S. Marcos, and R. van der Heijde, "Crystalline lens radii of curvature from Purkinje and Scheimpflug imaging," J. Vision 6, 1057-1067 (2006). [CrossRef]
  27. J. A. Izatt, M. R. Hee, E. A. Swanson, C. P. Lin, D. Huang, J. S. Schuman, C. A. Puliafito, and J. G. Fujimoto, "Micrometer-scale resolution imaging of the anterior eye in vivo with optical coherence tomography," Arch. Ophthalmol. 112, 1584-1589 (1994). [CrossRef] [PubMed]
  28. G. Baikoff, E. Lutun, and C. Ferraz, "Static and dynamic analysis of the anterior segment with optical coherence tomography," J. Cataract Refract. Surg. 30, 1843-1850 (2004). [CrossRef] [PubMed]
  29. C. K. Leung, W. M. Chan, C. Y. Ko, S. I. Chui, J. Woo, M. K. Tsang, and R. K. Tse, "Visualization of anterior chamber angle dynamics using optical coherence tomography," Ophthalmology 112, 980-984 (2005). [CrossRef] [PubMed]
  30. S. Radhakrishnan, A. M. Rollins, J. E. Roth, S. Yazdanfar, V. Westphal, D. S. Bardenstein, and J. A. Izatt, "Real-time optical coherence tomography of the anterior segment at 1310 nm," Arch. Ophthalmol. 119, 1179-1185 (2001). [PubMed]
  31. J. J. Kałużny, M. Wojtkowski, and A. Kowalczyk, "Imaging of the anterior segment of the eye by Spectra Optical Coherence Tomography," Opt. Appl. 32, 581-589 (2002).
  32. M. Wojtkowski, T. Bajraszewski, I. Gorczynska, P. Targowski, A. Kowalczyk, W. Wasilewski, and C. Radzewicz, "Ophthalmic imaging by spectral optical coherence tomography," Am. J. Ophthalmol. 138, 412-419 (2004). [CrossRef] [PubMed]
  33. B. J. Kałużny, A. Szkulmowska, M. Szkulmowski, T. Bajraszewski, A. Wawrocka, M. R. Krawczynski, A. Kowalczyk, and M. Wojtkowski, "Granular corneal dystrophy in 830-nm Spectral Optical Coherence Tomography," Cornea 27, 830-832 (2008). [CrossRef]
  34. B. J. Kaluzny, J. J. Kaluzny, A. Szkulmowska, I. Gorczynska, M. Szkulmowski, T. Bajraszewski, M. Wojtkowski, and P. Targowski, "Spectral Optical Coherence Tomography: A Novel Technique for Cornea Imaging," Cornea 25, 960-965 (2006). [PubMed]
  35. B. J. Kaluzny, J. J. Kaluzny, A. Szkulmowska, I. Gorczynska, M. Szkulmowski, T. Bajraszewski, P. Targowski, and A. Kowalczyk, "Spectral optical coherence tomography: a new imaging technique in contact lens practice," Ophthalmic Physiol. Opt. 26, 127-132 (2006). [CrossRef] [PubMed]
  36. V. Christopoulos, L. Kagemann, G. Wollstein, H. Ishikawa, M. L. Gabriele, M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, J. S. Duker, D. K. Dhaliwal, and J. S. Schuman, "In vivo corneal high-speed, ultra high-resolution optical coherence tomography," Arch. Ophthalmol. 125, 1027-1035 (2007). [CrossRef] [PubMed]
  37. B. J. Kaluzny, W. Fojt, A. Szkulmowska, T. Bajraszewski, M. Wojtkowski, and A. Kowalczyk, "Spectral optical coherence tomography in video-rate and 3D imaging of contact lens wear," Optom. Vis. Sci. 84, 1104-1109 (2007). [CrossRef] [PubMed]
  38. Y. Yasuno, V. D. Madjarova, S. Makita, M. Akiba, A. Morosawa, C. Chong, T. Sakai, K. P. Chan, M. Itoh, and T. Yatagai, "Three-dimensional and high-speed swept-source optical coherence tomography for in vivo investigation of human anterior eye segments," Opt. Express 13, 10652-10664 (2005). [CrossRef] [PubMed]
  39. C. Kerbage, H. Lim, W. Sun, M. Mujat, and J. F. de Boer, "Large depth-high resolution full 3D imaging of the anterior segments of the eye using high speed optical frequency domain imaging," Opt. Express 15, 7117-7125 (2007). [CrossRef] [PubMed]
  40. M. V. Sarunic, S. Asrani, and J. A. Izatt, "Imaging the ocular anterior segment with real-time, full-range Fourier-domain optical coherence tomography," Arch. Ophthalmol. 126, 537-542 (2008). [CrossRef] [PubMed]
  41. M. Miura, H. Mori, Y. Watanabe, M. Usui, K. Kawana, T. Oshika, T. Yatagai, and Y. Yasuno, "Three-dimensional optical coherence tomography of granular corneal dystrophy," Cornea 26, 373-374 (2007). [CrossRef] [PubMed]
  42. M. Miura, K. Kawana, T. Iwasaki, T. Kiuchi, T. Oshika, H. Mori, M. Yamanari, S. Makita, T. Yatagai, and Y. Yasuno, "Three-dimensional anterior segment optical coherence tomography of filtering blebs after trabeculectomy," J. Glaucoma 17, 193-196 (2008). [CrossRef] [PubMed]
  43. B. Potsaid, I. Gorczynska, V. J. Srinivasan, Y. Chen, J. Jiang, A. Cable, and J. G. Fujimoto, "Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second," Opt. Express 16, 15149-15169 (2008). [CrossRef] [PubMed]
  44. S. H. Yun, G. J. Tearney, J. F. de Boer, and B. E. Bouma, "Motion artifacts in optical coherence tomography with frequency-domain ranging," Opt. Express 12, 2977-2998 (2004). [CrossRef] [PubMed]
  45. M. Szkulmowski, A. Szkulmowska, I. Grulkowski, D. Szlag, A. Kowalczyk, and M. Wojtkowski, Nicolaus Copernicus University, Torun, Poland, are preparing a manuscript to be called "Quantitative flow velocity estimation in complex ambiguity free joint Spectral and Time domain Optical Coherence Tomography".
  46. A. Szkulmowska, M. Szkulmowski, A. Kowalczyk, and M. Wojtkowski, "Phase-resolved Doppler optical coherence tomography - limitations and improvements," Opt. Lett. 33, 1425-1427 (2008). [CrossRef] [PubMed]
  47. Y. Yasuno, S. Makita, T. Endo, G. Aoki, M. Itoh, and T. Yatagai, "Simultaneous B-M-mode scanning method for real-time full-range Fourier domain optical coherence tomography," Appl. Opt. 45, 1861-1865 (2006). [CrossRef] [PubMed]
  48. R. K. K. Wang, "In vivo full range complex Fourier domain optical coherence tomography," Appl. Phys. Lett. 90, 054103 (2007). [CrossRef]
  49. American National Standards Institute, Safe Use of Lasers (ANSI, 1993).
  50. M. Szkulmowski, A. Wojtkowski, T. Bajraszewski, I. Gorczynska, P. Targowski, W. Wasilewski, A. Kowalczyk, and C. Radzewicz, "Quality improvement for high resolution in vivo images by spectral domain optical coherence tomography with supercontinuum source," Opt. Commun. 246, 569-578 (2005). [CrossRef]
  51. P. Targowski, W. Gorczynska, M. Szkulmowski, M. Wojtkowski, and A. Kowalczyk, "Improved complex spectral domain OCT for in vivo eye imaging," Opt. Commun. 249, 357-362 (2005). [CrossRef]

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