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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding

Dawn Schafer, Emily A. Gibson, Evan A. Salim, Amy E. Palmer, Ralph Jimenez, and Jeff Squier  »View Author Affiliations


Optics Express, Vol. 17, Issue 8, pp. 6068-6073 (2009)
http://dx.doi.org/10.1364/OE.17.006068


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Abstract

A simple fabrication technique to create all silicon/glass microfluidic devices is demonstrated using femtosecond laser ablation and anodic bonding. In a first application, we constructed a cell counting device based on small angle light scattering. The counter featured embedded optical fibers for multiangle excitation and detection of scattered light and/or fluorescence. The performance of the microfluidic cell counter was benchmarked against a commercial fluorescence-activated cell sorter.

© 2009 Optical Society of America

OCIS Codes
(060.2310) Fiber optics and optical communications : Fiber optics
(170.1530) Medical optics and biotechnology : Cell analysis
(290.5850) Scattering : Scattering, particles

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 3, 2009
Revised Manuscript: March 18, 2009
Manuscript Accepted: March 18, 2009
Published: March 31, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Dawn N. Schafer, Emily A. Gibson, Evan A. Salim, Amy E. Palmer, Ralph Jimenez, and Jeff Squier, "Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding," Opt. Express 17, 6068-6073 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-17-8-6068


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