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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Multiplexed force measurements on live cells with holographic optical tweezers

Cecile O. Mejean, Andrew W. Schaefer, Eleanor A. Millman, Paul Forscher, and Eric R. Dufresne  »View Author Affiliations

Optics Express, Vol. 17, Issue 8, pp. 6209-6217 (2009)

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We describe open-loop and closed-loop multiplexed force measurements using holographic optical tweezers. We quantify the performance of our novel video-based control system in a driven suspension of colloidal particles. We demonstrate our system’s abilities with the measurement of the mechanical coupling between Aplysia bag cell growth cones and beads functionalized with the neuronal cell adhesion molecule, apCAM. We show that cells form linkages which couple beads to the underlying cytoskeleton. These linkages are intermittent, stochastic and heterogeneous across beads distributed near the leading edge of a single growth cone.

© 2009 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(090.1995) Holography : Digital holography

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: February 20, 2009
Revised Manuscript: March 21, 2009
Manuscript Accepted: March 29, 2009
Published: April 1, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Cecile O. Mejean, Andrew W. Schaefer, Eleanor A. Millman, Paul Forscher, and Eric R. Dufresne, "Multiplexed force measurements on live cells with holographic optical tweezers," Opt. Express 17, 6209-6217 (2009)

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