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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

On illumination schemes for wide-field CARS microscopy

I. Toytman, D. Simanovskii, and D. Palanker  »View Author Affiliations

Optics Express, Vol. 17, Issue 9, pp. 7339-7347 (2009)

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New system for a wide-field CARS microscopy is demonstrated, including two schemes of non-phase-matching illumination. Several advantages including high Stokes pulse energy, pulse-to-pulse stability and inherent synchronization between pump and Stokes pulses were brought by use of methane-filled Raman converter. Spatial resolution of the system with axially symmetric illumination, 0.5μm, was found to correspond to diffraction limit of the imaging objective. Selective sensitivity to lipid-rich myelin sheaths in the nerve tissue has been demonstrated and confirmed by comparison with histological samples stained with myelin-specific dye. Single-shot imaging capability of the system has been demonstrated with a speckling-free illumination on a monolayer of 3μm polystyrene beads.

© 2009 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(190.5650) Nonlinear optics : Raman effect
(190.5890) Nonlinear optics : Scattering, stimulated

ToC Category:

Original Manuscript: January 21, 2009
Revised Manuscript: March 5, 2009
Manuscript Accepted: April 7, 2009
Published: April 20, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

I. Toytman, D. Simanovskii, and D. Palanker, "On illumination schemes for wide-field CARS microscopy," Opt. Express 17, 7339-7347 (2009)

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