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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 9 — Jul. 6, 2010

Laminar optical tomography of the hemodynamic response in the lumbar spinal cord of rats

Nicolas Ouakli, Edgar Guevara, Simon Dubeau, Éric Beaumont, and Frédéric Lesage  »View Author Affiliations

Optics Express, Vol. 18, Issue 10, pp. 10068-10077 (2010)

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Intrinsic optical imaging (IOI) has emerged as a very powerful tool to assess neuronal function in small animals. Although it has been used extensively in the brain, its application to the spinal cord is rare. The inability of intrinsic optical techniques to resolve different depths and embedded gray matter hampers their capacity to distinguish larger vasculature contributions of hemodynamic signals originating from motoneuron and interneuron activation. Laminar optical tomography (LOT) is a recently-developed method that fills the gap left between IOI and diffuse optical imaging. With distinct source-detector separations, light that propagates deeper into tissues can be distinguished from light originating from the surface, providing depth sensitivity. In this work, LOT is investigated for the first time to image spinal cord activation with simultaneous IOI of the cortex in rats. Such proof of concept provides a powerful imaging modality to study spinal cord activation and disruption after injury.

© 2010 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3660) Medical optics and biotechnology : Light propagation in tissues

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 16, 2009
Revised Manuscript: March 11, 2010
Manuscript Accepted: April 28, 2010
Published: April 29, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Nicolas Ouakli, Edgar Guevara, Simon Dubeau, Éric Beaumont, and Frédéric Lesage, "Laminar optical tomography of the hemodynamic response in the lumbar spinal cord of rats," Opt. Express 18, 10068-10077 (2010)

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