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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 9 — Jul. 6, 2010

Measurement of the ratio of forward-propagating to back-propagating second harmonic signal using a single objective

Xiaoxing Han and Edward Brown  »View Author Affiliations


Optics Express, Vol. 18, Issue 10, pp. 10538-10550 (2010)
http://dx.doi.org/10.1364/OE.18.010538


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Abstract

In this paper, we present a method to determine, for the first time, the ratio of forward-propagating second harmonic (SHG) signal to back-propagating SHG signal (F/B) in vivo on the surface of intact tissue samples without any biopsy or tissue sectioning, using only epidetection (i.e., via a single objective lens). This method has the additional benefit of using the confocal detection apparatus already contained within common commercially available two-photon laser-scanning microscopes, and hence can allow the measurement of the SHG F/B ratio in vivo with minimal purchase of new equipment.

© 2010 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 23, 2010
Revised Manuscript: April 16, 2010
Manuscript Accepted: April 19, 2010
Published: May 5, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Xiaoxing Han and Edward Brown, "Measurement of the ratio of forward-propagating to back-propagating second harmonic signal using a single objective," Opt. Express 18, 10538-10550 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-10-10538


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