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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution

Waheb Bishara, Ting-Wei Su, Ahmet F. Coskun, and Aydogan Ozcan  »View Author Affiliations


Optics Express, Vol. 18, Issue 11, pp. 11181-11191 (2010)
http://dx.doi.org/10.1364/OE.18.011181


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Abstract

We demonstrate lensfree holographic microscopy on a chip to achieve ~0.6 µm spatial resolution corresponding to a numerical aperture of ~0.5 over a large field-of-view of ~24 mm2. By using partially coherent illumination from a large aperture (~50 µm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm2) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans.

© 2010 OSA

OCIS Codes
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: March 17, 2010
Revised Manuscript: May 3, 2010
Manuscript Accepted: May 6, 2010
Published: May 12, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Waheb Bishara, Ting-Wei Su, Ahmet F. Coskun, and Aydogan Ozcan, "Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution," Opt. Express 18, 11181-11191 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-11-11181


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