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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Ultra-fast, high-precision image analysis for localization-based super resolution microscopy

Tingwei Quan, Pengcheng Li, Fan Long, Shaoqun Zeng, Qingming Luo, Per Niklas Hedde, Gerd Ulrich Nienhaus, and Zhen-Li Huang  »View Author Affiliations


Optics Express, Vol. 18, Issue 11, pp. 11867-11876 (2010)
http://dx.doi.org/10.1364/OE.18.011867


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Abstract

Localization-based super resolution microscopy holds superior performances in live cell imaging, but its widespread use is thus far mainly hindered by the slow image analysis speed. Here we show a powerful image analysis method based on the combination of the maximum likelihood algorithm and a Graphics Processing Unit (GPU). Results indicate that our method is fast enough for real-time processing of experimental images even from fast EMCCD cameras working at full frame rate without compromising localization precision or field of view. This newly developed method is also capable of revealing movements from the images immediately after data acquisition, which is of great benefit to live cell imaging.

© 2010 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 29, 2010
Revised Manuscript: May 13, 2010
Manuscript Accepted: May 14, 2010
Published: May 20, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Tingwei Quan, Pengcheng Li, Fan Long, Shaoqun Zeng, Qingming Luo, Per Niklas Hedde, Gerd Ulrich Nienhaus, and Zhen-Li Huang, "Ultra-fast, high-precision image analysis for localization-based super resolution microscopy," Opt. Express 18, 11867-11876 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-11-11867


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