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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

A low-cost, portable, and quantitative spectral imaging system for application to biological tissues

Henry L. Fu, Bing Yu, Justin Y Lo, Greg M. Palmer, Thomas F. Kuech, and Nimmi Ramanujam  »View Author Affiliations

Optics Express, Vol. 18, Issue 12, pp. 12630-12645 (2010)

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The ability of diffuse reflectance spectroscopy to extract quantitative biological composition of tissues has been used to discern tissue types in both pre-clinical and clinical cancer studies. Typically, diffuse reflectance spectroscopy systems are designed for single-point measurements. Clinically, an imaging system would provide valuable spatial information on tissue composition. While it is feasible to build a multiplexed fiber-optic probe based spectral imaging system, these systems suffer from drawbacks with respect to cost and size. To address these we developed a compact and low cost system using a broadband light source with an 8-slot filter wheel for illumination and silicon photodiodes for detection. The spectral imaging system was tested on a set of tissue mimicking liquid phantoms which yielded an optical property extraction accuracy of 6.40 ± 7.78% for the absorption coefficient (µa) and 11.37 ± 19.62% for the wavelength-averaged reduced scattering coefficient (µs’).

© 2010 OSA

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 6, 2010
Revised Manuscript: April 23, 2010
Manuscript Accepted: May 15, 2010
Published: May 28, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Henry L. Fu, Bing Yu, Justin Y Lo, Greg M. Palmer, Thomas F. Kuech, and Nimmi Ramanujam, "A low-cost, portable, and quantitative spectral imaging system for application to biological tissues," Opt. Express 18, 12630-12645 (2010)

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