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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

A low-cost, portable, and quantitative spectral imaging system for application to biological tissues

Henry L. Fu, Bing Yu, Justin Y Lo, Greg M. Palmer, Thomas F. Kuech, and Nimmi Ramanujam  »View Author Affiliations


Optics Express, Vol. 18, Issue 12, pp. 12630-12645 (2010)
http://dx.doi.org/10.1364/OE.18.012630


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Abstract

The ability of diffuse reflectance spectroscopy to extract quantitative biological composition of tissues has been used to discern tissue types in both pre-clinical and clinical cancer studies. Typically, diffuse reflectance spectroscopy systems are designed for single-point measurements. Clinically, an imaging system would provide valuable spatial information on tissue composition. While it is feasible to build a multiplexed fiber-optic probe based spectral imaging system, these systems suffer from drawbacks with respect to cost and size. To address these we developed a compact and low cost system using a broadband light source with an 8-slot filter wheel for illumination and silicon photodiodes for detection. The spectral imaging system was tested on a set of tissue mimicking liquid phantoms which yielded an optical property extraction accuracy of 6.40 ± 7.78% for the absorption coefficient (µa) and 11.37 ± 19.62% for the wavelength-averaged reduced scattering coefficient (µs’).

© 2010 OSA

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 6, 2010
Revised Manuscript: April 23, 2010
Manuscript Accepted: May 15, 2010
Published: May 28, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Henry L. Fu, Bing Yu, Justin Y Lo, Greg M. Palmer, Thomas F. Kuech, and Nimmi Ramanujam, "A low-cost, portable, and quantitative spectral imaging system for application to biological tissues," Opt. Express 18, 12630-12645 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-12-12630


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References

  1. P. I. Tartter, J. Kaplan, I. Bleiweiss, C. Gajdos, A. Kong, S. Ahmed, and D. Zapetti, “Lumpectomy margins, reexcision, and local recurrence of breast cancer,” Am. J. Surg. 179(2), 81–85 (2000). [CrossRef] [PubMed]
  2. G. C. Balch, S. K. Mithani, J. F. Simpson, and M. C. Kelley, “Accuracy of intraoperative gross examination of surgical margin status in women undergoing partial mastectomy for breast malignancy,” Am. Surg. 71(1), 22–27, discussion 27–28 (2005). [PubMed]
  3. M. F. Dillon, E. W. Mc Dermott, A. O’Doherty, C. M. Quinn, A. D. Hill, and N. O’Higgins, “Factors affecting successful breast conservation for ductal carcinoma in situ,” Ann. Surg. Oncol. 14(5), 1618–1628 (2007). [CrossRef] [PubMed]
  4. F. J. Fleming, A. D. Hill, E. W. Mc Dermott, A. O’Doherty, N. J. O’Higgins, and C. M. Quinn, “Intraoperative margin assessment and re-excision rate in breast conserving surgery,” Eur. J. Surg. Oncol. 30(3), 233–237 (2004). [CrossRef] [PubMed]
  5. T. L. Huston, R. Pigalarga, M. P. Osborne, and E. Tousimis, “The influence of additional surgical margins on the total specimen volume excised and the reoperative rate after breast-conserving surgery,” Am. J. Surg. 192(4), 509–512 (2006). [CrossRef] [PubMed]
  6. L. Jacobs, “Positive margins: the challenge continues for breast surgeons,” Ann. Surg. Oncol. 15(5), 1271–1272 (2008). [CrossRef] [PubMed]
  7. C. Kotwall, M. Ranson, A. Stiles, and M. S. Hamann, “Relationship between initial margin status for invasive breast cancer and residual carcinoma after re-excision,” Am. Surg. 73(4), 337–343 (2007). [PubMed]
  8. J. E. Méndez, W. W. Lamorte, A. de Las Morenas, S. Cerda, R. Pistey, T. King, M. Kavanah, E. Hirsch, and M. D. Stone, “Influence of breast cancer margin assessment method on the rates of positive margins and residual carcinoma,” Am. J. Surg. 192(4), 538–540 (2006). [CrossRef] [PubMed]
  9. T. S. Menes, P. I. Tartter, H. Mizrachi, S. R. Smith, and A. Estabrook, “Touch preparation or frozen section for intraoperative detection of sentinel lymph node metastases from breast cancer,” Ann. Surg. Oncol. 10(10), 1166–1170 (2003). [CrossRef] [PubMed]
  10. M. C. Smitt, K. W. Nowels, M. J. Zdeblick, S. Jeffrey, R. W. Carlson, F. E. Stockdale, and D. R. Gfinet, “The importance of the lumpectomy surgical margin status in long-term results of breast conservation,” Cancer 76(2), 259–267 (1995). [CrossRef] [PubMed]
  11. J. Waljee, E. S. Hu, L. A. Newman, and A. K. Alderman, “Predictors of re-excision among women undergoing breast conserving surgery for cancer,” Ann. Surg. Oncol. 15(5), 1297–1298 (2008). [CrossRef] [PubMed]
  12. L. A. Carey, C. M. Perou, C. A. Livasy, L. G. Dressler, D. Cowan, K. Conway, G. Karaca, M. A. Troester, C. K. Tse, S. Edmiston, S. L. Deming, J. Geradts, M. C. Cheang, T. O. Nielsen, P. G. Moorman, H. S. Earp, and R. C. Millikan, “Race, breast cancer subtypes, and survival in the Carolina Breast Cancer Study,” JAMA 295(21), 2492–2502 (2006). [CrossRef] [PubMed]
  13. C. E. Cox, N. N. Ku, D. S. Reintgen, H. M. Greenberg, S. V. Nicosia, and S. Wangensteen, “Touch preparation cytology of breast lumpectomy margins with histologic correlation,” Arch. Surg. 126(4), 490–493 (1991). [PubMed]
  14. J. C. Cendán, D. Coco, and E. M. Copeland, “Accuracy of intraoperative frozen-section analysis of breast cancer lumpectomy-bed margins,” J. Am. Coll. Surg. 201(2), 194–198 (2005). [CrossRef] [PubMed]
  15. A. J. Creager, J. A. Shaw, P. R. Young, and K. R. Geisinger, “Intraoperative evaluation of lumpectomy margins by imprint cytology with histologic correlation: a community hospital experience,” Arch. Pathol. Lab. Med. 126(7), 846–848 (2002). [PubMed]
  16. S. A. McLaughlin, L. M. Ochoa-Frongia, S. M. Patil, H. S. Cody, and L. M. Sclafani, “Influence of frozen-section analysis of sentinel lymph node and lumpectomy margin status on reoperation rates in patients undergoing breast-conservation therapy,” J. Am. Coll. Surg. 206(1), 76–82 (2008). [CrossRef]
  17. J. Q. Brown, L. G. Wilke, J. Geradts, S. A. Kennedy, G. M. Palmer, and N. Ramanujam, “Quantitative optical spectroscopy: a robust tool for direct measurement of breast cancer vascular oxygenation and total hemoglobin content in vivo,” Cancer Res. 69(7), 2919–2926 (2009). [CrossRef] [PubMed]
  18. V. T. Chang, P. S. Cartwright, S. M. Bean, G. M. Palmer, R. C. Bentley, and N. Ramanujam, “Quantitative physiology of the precancerous cervix in vivo through optical spectroscopy,” Neoplasia 11(4), 325–332 (2009). [PubMed]
  19. R. Reif, M. S. Amorosino, K. W. Calabro, O. A’Amar, S. K. Singh, and I. J. Bigio, “Analysis of changes in reflectance measurements on biological tissues subjected to different probe pressures,” J. Biomed. Opt. 13(1), 010502 (2008). [CrossRef] [PubMed]
  20. R. A. Schwarz, W. Gao, C. Redden Weber, C. Kurachi, J. J. Lee, A. K. El-Naggar, R. Richards-Kortum, and A. M. Gillenwater, “Noninvasive evaluation of oral lesions using depth-sensitive optical spectroscopy,” Cancer 115(8), 1669–1679 (2009). [CrossRef] [PubMed]
  21. Z. Volynskaya, A. S. Haka, K. L. Bechtel, M. Fitzmaurice, R. Shenk, N. Wang, J. Nazemi, R. R. Dasari, and M. S. Feld, “Diagnosing breast cancer using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy,” J. Biomed. Opt. 13(2), 024012 (2008). [CrossRef] [PubMed]
  22. G. Zonios, L. T. Perelman, V. Backman, R. Manoharan, M. Fitzmaurice, J. Van Dam, and M. S. Feld, “Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo,” Appl. Opt. 38(31), 6628–6637 (1999). [CrossRef]
  23. I. J. Bigio, S. G. Bown, G. Briggs, C. Kelley, S. Lakhani, D. Pickard, P. M. Ripley, I. G. Rose, and C. Saunders, “Diagnosis of breast cancer using elastic-scattering spectroscopy: preliminary clinical results,” J. Biomed. Opt. 5(2), 221–228 (2000). [CrossRef] [PubMed]
  24. C. Zhu, G. M. Palmer, T. M. Breslin, J. Harter, and N. Ramanujam, “Diagnosis of breast cancer using diffuse reflectance spectroscopy: Comparison of a Monte Carlo versus partial least squares analysis based feature extraction technique,” Lasers Surg. Med. 38(7), 714–724 (2006). [CrossRef] [PubMed]
  25. C. Zhu, G. M. Palmer, T. M. Breslin, F. Xu, and N. Ramanujam, “Use of a multiseparation fiber optic probe for the optical diagnosis of breast cancer,” J. Biomed. Opt. 10(2), 024032 (2005). [CrossRef] [PubMed]
  26. G. M. Palmer and N. Ramanujam, “Monte Carlo-based inverse model for calculating tissue optical properties. Part I: Theory and validation on synthetic phantoms,” Appl. Opt. 45(5), 1062–1071 (2006). [CrossRef] [PubMed]
  27. G. M. Palmer, C. Zhu, T. M. Breslin, F. Xu, K. W. Gilchrist, and N. Ramanujam, “Monte Carlo-based inverse model for calculating tissue optical properties. Part II: Application to breast cancer diagnosis,” Appl. Opt. 45(5), 1072–1078 (2006). [CrossRef] [PubMed]
  28. T. M. Bydlon, S. A. Kennedy, L. M. Richards, J. Q. Brown, B. Yu, M. K. Junker, J. Gallagher, J. Geradts, L. G. Wilke, and N. Ramanujam, “Performance metrics of an optical spectral imaging system for intra-operative assessment of breast tumor margins,” Opt. Express 18(8), 8058–8076 (2010). [CrossRef] [PubMed]
  29. L. G. Wilke, J. Q. Brown, T. M. Bydlon, S. A. Kennedy, L. M. Richards, M. K. Junker, J. Gallagher, W. T. Barry, J. Geradts, and N. Ramanujam, “Rapid noninvasive optical imaging of tissue composition in breast tumor margins,” Am. J. Surg. 198(4), 566–574 (2009). [CrossRef] [PubMed]
  30. C. C. Yu, C. Lau, G. O’Donoghue, J. Mirkovic, S. McGee, L. Galindo, A. Elackattu, E. Stier, G. Grillone, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Quantitative spectroscopic imaging for non-invasive early cancer detection,” Opt. Express 16(20), 16227–16239 (2008). [CrossRef] [PubMed]
  31. J. Y. Lo, B. Yu, H. L. Fu, J. E. Bender, G. M. Palmer, T. F. Kuech, and N. Ramanujam, “A strategy for quantitative spectral imaging of tissue absorption and scattering using light emitting diodes and photodiodes,” Opt. Express 17(3), 1372–1384 (2009). [CrossRef] [PubMed]
  32. B. Yu, J. Y. Lo, T. F. Kuech, G. M. Palmer, J. E. Bender, and N. Ramanujam, “Cost-effective diffuse reflectance spectroscopy device for quantifying tissue absorption and scattering in vivo,” J. Biomed. Opt. 13(6), 060505 (2008). [CrossRef]
  33. S. Prahl, Mie Scattering Program, Oregon Medical Laser Center, 2005, http://omlc.ogi.edu/software/mie/ .
  34. J. E. Bender, K. Vishwanath, L. K. Moore, J. Q. Brown, V. Chang, G. M. Palmer, and N. Ramanujam, “A robust Monte Carlo model for the extraction of biological absorption and scattering in vivo,” IEEE Trans. Biomed. Eng. 56(4), 960–968 (2009). [CrossRef] [PubMed]
  35. G. M. Palmer and N. Ramanujam, “Use of genetic algorithms to optimize fiber optic probe design for the extraction of tissue optical properties,” IEEE Trans. Biomed. Eng. 54(8), 1533–1535 (2007). [CrossRef] [PubMed]

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