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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Image processing guided analysis for estimation of bacteria colonies number by means of optical transforms

Igor Buzalewicz, Katarzyna Wysocka-Król, and Halina Podbielska  »View Author Affiliations

Optics Express, Vol. 18, Issue 12, pp. 12992-13005 (2010)

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A novel method for evaluation of bacterial colonies number (Colony Forming Units - CFU), is described. Proposed algorithm, based on the Mellin transform, allows the CFU evaluation, invariant for the spatial orientation and scale changes. The proposed method involves image recording of bacteria grown in Petri dishes, calculation of the Fourier spectrum followed by coordinates transformation, and determination of the Mellin transform. It was proved that there is a high correlation between CFU and maxima of Mellin spectra. The method was practically implemented for evaluation of antibacterial activity of silver-based nanomaterials and the effect of an additional laser light irradiation.

© 2010 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 2, 2010
Revised Manuscript: May 8, 2010
Manuscript Accepted: May 11, 2010
Published: June 2, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Igor Buzalewicz, Katarzyna Wysocka-Król, and Halina Podbielska, "Image processing guided analysis for estimation of bacteria colonies number by means of optical transforms," Opt. Express 18, 12992-13005 (2010)

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