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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 11 — Aug. 25, 2010

Improved sensitivity for two-photon frequency-domain lifetime measurement

Arnold D. Estrada and Andrew K. Dunn  »View Author Affiliations

Optics Express, Vol. 18, Issue 13, pp. 13631-13639 (2010)

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We demonstrate a method to improve the measurement sensitivity of two-photon frequency-domain lifetime measurements in poor signal to background conditions. This technique uses sinusoidal modulation of the two-photon excitation source and detection of the second harmonic of the modulation frequency that appears in the emission. Additionally, we present the mathematical model which describes how the observed phase shift and amplitude demodulation factor of two-photon phosphorescence emission are related to the phosphorescence lifetime and modulation frequency. We demonstrate the validity of the model by showing the existence of new frequency terms in the phosphorescence emission generated from the quadratic nature of two-photon absorption and by showing that the phase shift and demodulation match theory for all frequency components.

© 2010 OSA

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Nonlinear Optics

Original Manuscript: February 26, 2010
Revised Manuscript: May 28, 2010
Manuscript Accepted: May 29, 2010
Published: June 10, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Arnold D. Estrada and Andrew K. Dunn, "Improved sensitivity for two-photon frequency-domain lifetime measurement," Opt. Express 18, 13631-13639 (2010)

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