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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 11 — Aug. 25, 2010

Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation

Jeffrey J. Field, Ramón Carriles, Kraig E. Sheetz, Eric V. Chandler, Erich E. Hoover, Shane E. Tillo, Thom E. Hughes, Anne W. Sylvester, David Kleinfeld, and Jeff A. Squier  »View Author Affiliations

Optics Express, Vol. 18, Issue 13, pp. 13661-13672 (2010)

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A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores—rhodamine B, a red fluorescent protein, and CdSe quantum dots—while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples.

© 2010 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(320.5540) Ultrafast optics : Pulse shaping
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: April 2, 2010
Revised Manuscript: May 28, 2010
Manuscript Accepted: June 4, 2010
Published: June 10, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Jeffrey J. Field, Ramón Carriles, Kraig E. Sheetz, Eric V. Chandler, Erich E. Hoover, Shane E. Tillo, Thom E. Hughes, Anne W. Sylvester, David Kleinfeld, and Jeff A. Squier, "Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation," Opt. Express 18, 13661-13672 (2010)

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