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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 11 — Aug. 25, 2010

A compact acousto-optic lens for 2D and 3D femtosecond based 2-photon microscopy

Paul A. Kirkby, K. M. Naga Srinivas Nadella, and R. Angus Silver  »View Author Affiliations

Optics Express, Vol. 18, Issue 13, pp. 13720-13744 (2010)

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We describe a high speed 3D Acousto-Optic Lens Microscope (AOLM) for femtosecond 2-photon imaging. By optimizing the design of the 4 AO Deflectors (AODs) and by deriving new control algorithms, we have developed a compact spherical AOL with a low temporal dispersion that enables 2-photon imaging at 10-fold lower power than previously reported. We show that the AOLM can perform high speed 2D raster-scan imaging (>150 Hz) without scan rate dependent astigmatism. It can deflect and focus a laser beam in a 3D random access sequence at 30 kHz and has an extended focusing range (>137 μm; 40X 0.8NA objective). These features are likely to make the AOLM a useful tool for studying fast physiological processes distributed in 3D space

© 2010 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(230.1040) Optical devices : Acousto-optical devices
(320.2250) Ultrafast optics : Femtosecond phenomena

ToC Category:

Original Manuscript: March 15, 2010
Revised Manuscript: June 1, 2010
Manuscript Accepted: June 6, 2010
Published: June 11, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Paul A. Kirkby, K. M. Naga Srinivas Nadella, and R. Angus Silver, "A compact acousto-optic lens for 2D and 3D femtosecond based 2-photon microscopy," Opt. Express 18, 13720-13744 (2010)

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