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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 11 — Aug. 25, 2010

Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy

Liang Gao, Robert T. Kester, Nathan Hagen, and Tomasz S. Tkaczyk  »View Author Affiliations


Optics Express, Vol. 18, Issue 14, pp. 14330-14344 (2010)
http://dx.doi.org/10.1364/OE.18.014330


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Abstract

A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 × 285 × 60 (x, y, λ). The spatial resolution is ~0.45 µm with a FOV of 100 × 100 µm2. The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval ~3.3 nm. The channel’s spectral resolution is ~8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Imaging Systems

History
Original Manuscript: April 6, 2010
Revised Manuscript: June 14, 2010
Manuscript Accepted: June 14, 2010
Published: June 21, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Liang Gao, Robert T. Kester, Nathan Hagen, and Tomasz S. Tkaczyk, "Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy," Opt. Express 18, 14330-14344 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-14-14330


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