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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 3 — Feb. 10, 2010

Nonlinear optical imaging of lyotropic cholesteric liquid crystals

Ariane Deniset-Besseau, Paulo De Sa Peixoto, Gervaise Mosser, and Marie-Claire Schanne-Klein  »View Author Affiliations

Optics Express, Vol. 18, Issue 2, pp. 1113-1121 (2010)

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We use nonlinear optical microscopy combining Second Harmonic Generation (SHG) microscopy and Two-Photon Excited Fluorescence (2PEF) signals to characterize collagen lyotropic liquid crystals. We show that SHG signals provide highly contrasted images of the three-dimensional texture of cholesteric patterns with submicrometer lateral resolution. Moreover, simultaneous recording of the 2PEF signal enables in situ quantitative mapping of the molecular concentration and its correlation with the observed textures. We apply this technique to the characterization of biomimetic textures obtained in concentrated collagen liquid solutions. We successfully image biologically relevant organizations that are similar to the collagen organization found as a stabilized state in compact bones.

© 2010 OSA

OCIS Codes
(160.3710) Materials : Liquid crystals
(190.4180) Nonlinear optics : Multiphoton processes
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:

Original Manuscript: November 30, 2009
Revised Manuscript: December 23, 2009
Manuscript Accepted: December 30, 2009
Published: January 8, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Ariane Deniset-Besseau, Paulo De Sa Peixoto, Gervaise Mosser, and Marie-Claire Schanne-Klein, "Nonlinear optical imaging of lyotropic cholesteric liquid crystals," Opt. Express 18, 1113-1121 (2010)

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