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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 3 — Feb. 10, 2010

Collecting back-reflected photons in photoacoustic microscopy

Hao F. Zhang, Jing Wang, Qing Wei, Tan Liu, Shuliang Jiao, and Carmen A. Puliafito  »View Author Affiliations


Optics Express, Vol. 18, Issue 2, pp. 1278-1282 (2010)
http://dx.doi.org/10.1364/OE.18.001278


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Abstract

Since the photoacoustic effect relies only on the absorbed optical energy, the back-reflected photons from samples in optical-resolution photoacoustic microscopy are usually discarded. By employing a 2 × 2 single-mode fiber optical coupler in a laser-scanning optical-resolution photoacoustic microscope for delivering the illuminating laser light and collecting the back reflected photons, a fiber-optic confocal microscope is integrated with the photoacoustic microscope. Thus, simultaneous multimodal imaging can be achieved with a single light source and images from the two modalities are intrinsically registered. Such capabilities are demonstrated in imaging both phantoms and small animals in vivo.

© 2010 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.5120) Imaging systems : Photoacoustic imaging
(170.1790) Medical optics and biotechnology : Confocal microscopy

ToC Category:
Microscopy

History
Original Manuscript: November 30, 2009
Revised Manuscript: December 31, 2009
Manuscript Accepted: January 4, 2010
Published: January 11, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Hao F. Zhang, Jing Wang, Qing Wei, Tan Liu, Shuliang Jiao, and Carmen A. Puliafito, "Collecting back-reflected photons in photoacoustic microscopy," Opt. Express 18, 1278-1282 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-2-1278


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