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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 3 — Feb. 10, 2010

Instantaneous spatial light interference microscopy

Huafeng Ding and Gabriel Popescu  »View Author Affiliations


Optics Express, Vol. 18, Issue 2, pp. 1569-1575 (2010)
http://dx.doi.org/10.1364/OE.18.001569


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Abstract

We present Instantaneous Spatial Light Interference Microscopy (iSLIM) as a new quantitative phase method that combines the benefits of white light illumination in Zernike’s phase contrast microscopy and phase stability associated diffraction phase microscopy. iSLIM is implemented as an add-on module to a commercial phase contrast microscope, and enables new features to quantitative phase imaging: diminished speckle effects due to white light illumination, multimodal investigation potential due to overlaying with other modalities of the microscope (e.g. fluorescence, DIC, phase contrast), and spectroscopic potential due to the broad band light. We show proof of principle results by multicolor phase imaging of microsphere and red blood cells, and dynamic imaging of nanoscale cell membrane fluctuations.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: November 2, 2009
Revised Manuscript: December 18, 2009
Manuscript Accepted: January 7, 2010
Published: January 13, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Huafeng Ding and Gabriel Popescu, "Instantaneous spatial light interference microscopy," Opt. Express 18, 1569-1575 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-2-1569


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References

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